Reverse transcriptase

Similar to Tuberculosis and HIV, tinselsparkleitis shares similar destructive effects on the human body. Tinselsparkleitis is a viral infection caused by the spread of bodily fluids and having close contact with the infected victims. When a patient is infected, it could take up to two years for the victims to show any form of the disease and its symptoms. The beginning signs of the infection are flu like symptoms including fever, sore throat, fatigue, and vomiting with additional a small red and…

Question 6: List the principles of chemotherapeutic resistance. Write short note on the one you consider most important. Answer: Resistance describes reduction in effectiveness of drug to perform its normal function. It is the ability of microbes; bacterial, fungi, virus to resist the toxic effects of drugs and grow in the presence of the same concentration of drug that will normally kill or limit its growth. The following are the principles of chemotherapeutic resistance: • Pharmaceutic factors…

EBOLA VIRUS According to a CDC article on 10/03/2014, Ebola was first discovered in 1976 near the Ebola River in what is now the Democratic Republic of Congo. Since then, outbreaks have appeared in Africa and even reached other continents such as Europe and America. Ebola, previously known as Ebola hemorrhagic fever, is a rare and deadly disease caused by infection with one of the Ebola virus strains. The virus can cause disease in humans and nonhuman primates such as monkeys, gorillas and…

TERMINOLOGY CLINICAL CLARIFICATION Advanced stage of immunosuppression caused by the HIV virus 4 Characterized by abnormally low CD4+ T cell count {1 month in duration), or bronchitis, pneumonitis, or esophagitis Invasive cervical carcinoma confirmed by biopsy Lymphoma {Burkitt, immunoblastic, or primary central nervous system) Disseminated or extra pulmonary coccidioidomycosis Disseminated or extra pulmonary histoplasmosis Progressive multifocalleukoencephalopathy (PML) HIV-related…

Laboratory Diagnosis Monitoring Parameters • Tourniquet Test Acts as an emergency diagnostic tool whereby formation of more than 20 petechiae per square inch indicates a positive tourniquet test. A circle of 2.5 cm in diameter may be drawn and the number of petechiae in it is counted. More than five petechiae indicates positive test. • Complete Blood Cell Count Blood tests present with lowered total white blood cell, platelet and neutrophil counts and a 20% or more increase in haematocrit…

HIV, the human immunodeficiency virus. HIV damages and kills cells that are in the body 's immune system. There are different stages of HIV, but the most advanced stage is AIDS. AIDS stands for acquired immunodeficiency syndrome. HIV is spread through unprotected sex, sharing needles, and blood contact, all with an infected person. In this paper I will discuss HIV/AIDS in women, also the testing and prevention of the infection for women. Although both men and women can become infected with…

The project that I chose for the Baroness to fund was proposed by John, Anne, Adam, and Heather and was title “Integration of Lignin-Degrading Enzyme in Cow Rumen”. This project is seeking to generate a recombinant E. coli that degrades lignin into digestible compounds that the cow can utilize as energy source. This project was particularly useful in that it is seeking to take advantage of an energy source that is a carbon rich nutrient, thus allowing for an increase in efficiency of digestion…

1) Alcaligenes faecalis is a Gram-negative coccobacilli that is motile, oxidase positive, catalase positive, non-spore-forming, non-fermenting, slow growing, and citrate positive obligate aerobe (Mahon et al., 2014) (Sachdeva et al., 1963). Its colony morphology is flat, thin, and rough with irregular edging (Mahon et al., 2014) and the flagella varies between the usual shape versus the curly structure, however, both shapes do not exist on the same individual bacteria (Leifson, 1960). The…

Viruses are found wherever there is life and have probably existed since living cells evolved, but the origin of viruses is unclear because they do not form fossils. Instead, researchers have resorted to using molecular techniques to compare DNA of viruses to other organisms. Studies at the molecular level have revealed relationships between viruses infecting organisms from each of the three domains of life and viral proteins that pre-date the divergence of life and thus the last universal…

Real-time RT-PCR 1) Treat the embryos with compounds for 3 h 2) Extract the RNA 3) Isolate the total RNA from ten treated embryos using an RNeasy Mini kit (QIAgen) 4) Then treat with DNase, 5) Using Moloney murine leukemia virus (MMLV) Reverse Transcriptase (Promega) reverse-transcribe 2 μg of RNA. 6) Quantify the mRNA level using an Eppendorf Real-Time Cycler plus β-actin as a reference. The primer sequences were as follows: p53: 50-ATGTGGTGCCTGCCTCAGA-30 (sense)…