Restriction enzyme

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    protein structure prediction is one among the foremost necessary goals pursued by bioinformatics and theoretical chemistry; it's extremely necessary in medication (for example, in drug design) and biotechnology (for example, within the style of novel enzymes). each 2 years, the performance of current ways is assessed within the CASP experiment (Critical Assessment of Techniques for protein Structure…

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    Abstract: Catalase is an iron porphyrin enzyme present in high concentrations in humans and animals liver tissues. It is a peroxisomal enzyme that is responsible for destruction of toxic H2O2 released during certain metabolic processes.The activity of catalase can be determined by measuring the decrease in H2O2 concentration which is the substrate. Introduction: An enzyme is a large protein that acts as a biological catalyst which changes the rate of a reaction. It provides an active site…

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    What Is Laccases?

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    multi copper oxidases (Mate and Alcalde, 2016a).They catalyze the oxidation of compounds that are coupled with the four-electron reduction of "O" _"2" "to" "H" _"2" "O" . They are regarded as eco-friendly enzymes. Laccases have been first discovered in 1883, which makes it one of the oldest enzymes ever observed (Kunamneni et al., 2008).Laccases are found in a diversity of fungi, bacteria and higher plant species (Santhanam et al., 2011). These laccases are involved in a degree of…

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    Oxysterol Essay

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    The role of oxysterols in human body and disease 1. Introduction Oxysterols are 27-carbon derivatives of cholesterol created by spontaneous or enzymatic oxidation (Figure 1, (Brown and Jessup, 2009)). The oxidation of cholesterol occurs either on the side chain or on the sterol core. Side chain oxidation generates, e.g., 24-hydroxycholesterol (24-HC), 25-hydroxycholesterol (25-HC) or 27-hydroxycholesterol (27-HC) while the core oxidation gives rise to ring oxysterols, mostly ring-B oxysterols,…

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    Lipids In Milk Lab Report

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    Introduction The purpose of this lab was to identify the lipids in an egg yolk and to identify the amount of lipids in milk [1]. To extract the lipids from the egg yolk, the egg yolk needed to be combined with an organic solvent such as a mixture of CHCl3/MeOH. An organic solvent was needed to carry the extracted lipids up the TLC plate because the plate was polar, due to the presence of silica gel (silanol groups). To compare relatively similar lipids to the ones found in the egg yolk, the egg…

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    Alkaline Hydrolysis Essay

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    substrate concentration (%N × 6.25), (ii) enzyme-substrate ratio (E/S) in % or in activity units per kg N × 6.25, (iii) pH, (iv) temperature, and (v) time (Adler-Nissen, 1984). Generally, there is an optimum combination of both pH and temerature, where the enzyme shows the highest activity. Physicochemical conditions of the hydrolysis reaction such as temperature, pH and enzyme/substrate ratio must be adjusted to optimize the activity of the proteolytic enzyme (Kim & Wijesekara, 2010; Santos,…

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    Abstract The purpose of this investigation is to determine whether the temperature can affect the reaction rate of enzyme activity. The enzyme activity was measured by five different temperatures, separated in tubes which later got measured by their height of foam after 10 minutes. Freshly-prepared potato juice was used as the enzyme for catalase. Since temperature is measured by the kinetic energy of molecules, the higher the temperature, the more movement of molecules. The lowest temperature…

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    Amylase Biology Lab Report

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    The Effect of Change in Temperature Conditions on Enzymes Bacillus licheniformis Amylase and Aspergillus oryzae Amylase Over Time ____________________________________ Roxanna Flores PID: 5199946 Lab Section U26 Group Members: Roudabyna Taleb Abstract This laboratory was performed in order to be able to determine the effect of temperature over time on bacterial amylase Bacillus licheniformis and fungal amylase Aspergillus oryzae. Bacterial amylase was introduced into four singular test tubes…

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    Acid in the stomach functions to kill bacteria, and aid digestion by solubilizing food. The acid is also important to establish the optimal pH (between 1.8 and 3.5) for the function of the digestive enzyme pepsin. Stimulation of acid secretion involves the translocation of H+/K+-ATPases to the apical membrane of the parietal cell. When the cell is resting (not stimulated), H+/K+-ATPases are located in vesicles inside the cell. When the cell is stimulated…

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    Gummy Bear Experiment

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    For our diffusion experiment our group decided to work with salt water and gummy bears. Our experiment tested the weight of the gummy bears per salt dilutions. Our tests included 1g 2.5g 5g, 1.5g, and 10g of salt diluted with water. We knew that the higher the concentration of salt diluted into the water the heavier the diffused gummy bear would be, therefore we measure weight as our numerical data. My hypothesis was that the 10g dilution of salt would have the gummy bear with the largest…

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