Restriction enzyme

introduce the basics of enzymes. The topic is important because they are in every living cell and because of enzymes chemistry is allowed in the cell. They do this by providing the energy needed for chemical bonds to break down and lets molecules interact with each other. For part 1 of the lab my hypothesis was if you increase the concentration, then the absorbance will also increase. For part 2 section A my hypothesis was if you increase the temperature, then the enzyme reaction time will also…

3.0 RESULTS AND DISCUSSION 3.1: Effect of Storage Conditions on Egg Weight Tables 1- 2 show the results of effect of storage conditions on eggs weight (chicken eggs and quail eggs). The weight of chicken eggs stored at ambient condition (control), moist sawdust, refrigerator and those that were dipped in oil reduced from 63.61-60.03g, 63.11-60.99g, 61.12-59.41g and 66.12-65.79g respectively. While the weight of quail eggs stored at ambient condition (control), moist sawdust, refrigerator and…

Characterization of the oxidized amylose and the linalool/oxidized amylose inclusion complex. The high water solubility of oxidized amylose can broaden the applications in water environment.14, 32 The Table 1 shows the properties of amylose and oxidized amylose. As expected, the solubility of oxidized amyloses in water is highly improved due to the introduced carboxyl and carbonyl groups. And it increases greatly with the increase of carboxyl content. The results suggest that due to the two-step…

Vmax value when compared to other 3 substrates (Table 1). Table 1: Substrate specificity of PPO enzyme Substrates Specific activity (units mg-1 protein) Km (µM) Vmax (abs min-1) Catechol 2300 4.12 X 10-3 3020 L-DOPA 430 16.40 X 10-3 990 L-Tyrosine 1930 6.12 X 10-3 2930 P-Cresol 2110 5.01 X 10-3 2983 Effect of Inhibitors The effects of various compounds on the PPO activity were provided in Tables 2. Enzyme activity was markedly…

INVESTIGATE AN ASPECT OF REDOX SYSTEM/REACTION Research Question: Does a higher voltage lead to more mass on graphite? INTRODUCTION Hypothesis: Higher voltage leads to more mass amassed on graphite electrode. Electrolysis- the process in which electric current passes through a solution to effect a chemical change. In this case there is copper, copper sulphate and graphite. In a electrolysis there is an electrolyte which is the copper sulphate, the anode which is the graphite. This is the…

and temperature on the caseinolytic activity of crude protease were done. Stability towards temperature and pH were also evaluated. The pH and temperature activity curves and other stability studies suggested the possible presence of one proteolytic enzyme in the latex of Vallaris solanacea. In the present study, separation and purification of the cysteine protease…

OBJECTIVE For the first week of the lab, the objectives were to isolate and test for the presence of major components of milk, including proteins, carbohydrates, fats, vitamins, and minerals and to determine the presence of the various components of milk in the various fractions using qualitative tests for these substances. For the second week of the lab, the there were three objectives that related to the procedure: to determine the calcium concentration of milk using the technique of…

Introduction The ferric chloride test is used to determine the presence of phenols in a given sample or compound (for instance natural phenols in a plant extract). Enols, hydroxamic acids, oximes, and sulfinic acids give positive results as well. The ferric chloride test can be used to detect metabolites in urine in case of inborn error of metabolism such as phenylketonuria. In addition,the Ferric Chloride test can be used to detect salicylates in urine after an aspirin overdose.1 Background…

The synthesis of Aa-g-poly(MAA) involve the optimization of various reaction conditions like initiator concentration, monomer concentration, crosslinker concentration, solvent concentration and pH. After the optimization of various reaction parameters the replications were carried-out in triplicates for the reproducibility of the results and statistical of the results obtained. It was performed using the Statistical Package for Social Science (SPSS) version 10. Statistical results of the…

composite has been investigated. For that purpose, the PMMA samples with Al2O3 additive prepared with different percentages(0,15,25,35 and 45) wt.% and different thickness. The experimental results showed that the D.C electrical conductivity increased with increasing the alumina concentrations and temperature. Also the activation energy change with increasing of additional alumina. The dielectric constant, dielectric loss, A.C electrical conductivity are changed with change the concentration of…