Restriction enzyme

Purpose The main purpose of this experiment to learn about the organic molecule of the substances that we use daily with the common foods. The organic molecule that we use for this lab are monosaccharides of sugar, protein, starch, lipids, nucleic acids, and carbohydrates. Introduction Carbohydrates are the organic compounds that are organized as the ring structures and always compose the carbon, hydrogen, and oxygen atoms that represent by the structure of H2O. The carbohydrates are…

INTRODUCTION: In this lab, proper laboratory technique was implemented to have the experience in the lab. Furthermore, the unknown solution needed to be investigated, to determine the solution’s macromolecules. Three methods were comprised to determine the unknown but a set of other solution were used to help as indicators to match the unknown solution’s results. It consisted of biuret, iodine and benedict solution. Iodine solution is used to determine if the source had starch or glycogen.…

to NAD+ by CMI(9) . By coupling the associated metabolisms of these two enzymes NADH is produced using light and water as substrates with oxygen as the only…

To successfully co-reconstitute both enzymes into a single liposome it was necessary to first conduct detergent screening experiments for the reconstitution of each enzyme independently. To establish the most suitable detergent and detergent concentration, the magnitude of PMF generation along with enzymatic activity was measured for each reconstituted enzyme. Of the detergents tested, the zwitterionic surfactant CHAPS was found most suitable for…

Activity of Enzymes within a Potato Independent Variable: Temperature Dependent Variable: Visible amount of Fizz Constant: Size of Potato being used Introduction We have studied information about chemical reactions and how a catalyst is used to speed up reactions. We have learned that enzymes (proteins) in eukaryotic cells work as a catalyst to make this possible. Each enzyme in a eukaryotic cells has specific molecules it binds to, as they have a specific frame work. This…

Hypothesis: Aerobic Respiration: If a phosphate buffer, dichlorophenol-indophenol and succinate are added to a mitochondrial extract then the succinate molecules will bind to an enzyme complex (succinate dehydrogenase). Because the enzyme and FAD are a part of the same complex, initiating succinate oxidation, this reaction reduces FAD to FADH2. Thus the oxidation of succinate to furmarate. Anaerobic Respiration: If a solution of yeast and glucose is combined and placed in different incubation…

• What are the strategic choices available? Biocon India Group is a Bangalore-based organization that began in 1978 with the enzyme business. An enzyme, by the way, is a substance produced by a living organism which acts as a catalyst to bring about a specific biochemical reaction. That said, the Biocon India realized that growth is imminent and various strategic choices are available. The first strategic choice available to Managing Director, Kiran Mazumdar-Shaw’s Biocon India is expansion…

2. What are the differences between deep freezer storage and cryo-storage methods? Deep freezing storage and cryo-storage, methods are some of the methods frequently used to preserve microorganisms for a long term storage. Both methods use extremely low temperature to ensure the biological material remains genetically stable and metabolically inert. However both methods differ slightly from each other. Cryo-storage, also known as cryopreservation, uses liquid nitrogen to freeze the…

Chemical Reactions I Purpose The purpose of this experiment was to test and see how four different chemical reaction worked. The four different chemical reactions are formation, decomposition, single-replacement, and double-replacement. The hypothesis of this experiment was that in each of the different reactions something would happen to the reactant whether it be color, mass, temperature, or pressure. II Equipment For this experiment Late Nite lab was used. In Late Nite Lab the equipment…

Emulsifying properties of aqueous extract of Spirulina in ice cream Table 7 presents the results of the developed O/W and W/O emulsifiers of the developed ice cream formulations. The O/W activities of the formulations of aqueous extract of Spirulina presented higher ativity when compared to the standard formulation, the formulations without emulsifier (F2) and without chantilly (F3) showed no significant difference (p0.05) on the surface tensions values found in the analyzes. The formulations…