Restriction enzyme

Stromelysin-1 Stromelysin-1 is used to identify risk of developing CAD. Stromelysin-1 or matrix metalloproteinase-3 (MMP-3) is a matrix remodeling enzyme which degrades collagen, proteoglycans, gelatin and elastin. Therefore MMP-3 affects the matrix composition, which affects lipid accumulation, vascular remodeling and plaque instability resulting in cardiovascular disease. Human MMP-3 gene is found…

forensic science to estimate age –at-death since the mtDNA are altered by mutation. One way to slow down the aging process due to ROS production in mitochondria is reducing the available calorie to the mitochondria ETC for the electrons. Dietary restriction by the nuclear DNA inhibits the accumulation of both form of mtDNA damage. This mtDNA mutation is the result of endosymbiosis, since mitochondria lost many of its organelles to the nuclear DNA, it can’t protect itself from the over production…

and dibasic potassium. Phosphorus occurs naturally in every cell in the body and is used for repair and growth of cells and tissues. As an organic and inorganic phosphate, it has a significant biochemical function in the body creating metabolic and enzyme responses in almost all organs where it influences the steadiness of calcium levels, neutralizes acidic and basic ions in a solution and has a role in…

Everyone has their own problems they have to deal with everyday. Some people have more difficult problems to deal with than others. For example some peoples problems maybe to study for an exam. Others may just be trying to get through another day. People with disorders like anorexia struggle to live everyday. Anorexia is a disorder that means people won 't eat for days to the point where they almost die because they psychologically believe that their fat. Many people in the world that have this…

extend your life, but to look and feel younger during it, you would assume they were certifiable or, at the very least, after your money. But if there a scientifically proven strategy to do so, would you believe it then? This biohack, called Calorie Restriction (CR), has been shown to extend the life span in primates and rodent species reliably over the past 20 years of research. The best news is, there is very good reason to believe that these same benefits can be gained in the human life span…

Enzymes speed up the rates of most biochemical reactions by helping reactants interact with each other. Since enzymes are highly specific for a specific reaction, they only catalyze one or a few types of reaction. One of important enzymes in human body and many other animals is amylase that hydrolyzes starch, a major part of human diet for many people in the world. There…

Fermentation Rate in Different Sugars Abstract: This experiment is designed by students after studying the process of cellular respiration and fermentation. It demonstrates students’ understanding over the materials and utilization of the lab setting. In this experiment, the primary focus will on the speed of fermentation in different substances (sugars) in the same condition (temperature, volume, amount of yeast). Each solution will be mix with a fix amount of yeast and put in fermentation tube…

The purpose of conducting this experiment was to explore how different factors affect the reaction rate of enzymes reacting with their corresponding substrates in order to learn more about how enzymes function in different environments. The independent variables investigated in this experiment were the concentration of different substrates, the temperature of the environment, and the effect of a catalyst on the reaction rate. The dependent variable for all of the investigations was the time it…

Alkaline phosphatase (AP) is a homodimeric enzyme complex that is commonly found in a wide range of organisms, from bacteria to all tissues of the human body. AP is a zinc metalloenzyme (1), in which metal ions play a key role in the regulation of catalytic activities and stabilization of enzyme-substrate complex. As proposed by Gettins and Coleman using NMR studies (10), each active site of AP comprises of three metal binding sites, which acknowledged as M1, M2, and M3. Two zinc ions bind to…

This bacteria had a positive catalase test meaning that it contains the enzyme catalase and was able to turn the hydrogen peroxide into water and oxygen and that this bacteria uses aerobic respiration. When plated on a mannitol/salt plate the bacteria was able to grow on the plate meaning that it has a resistance to high salt…