Restriction enzyme

In this lab, many materials were used including, EcoRl restriction enzyme, DNA from the crime scene and subjects 1 through 5, centrifuge, water bath, DNA loading dye, Electrophoresis chamber, TAE buffer, and a fast blast stain. The EcoRl restriction enzyme is the enzyme that was used in order to cut the DNA at specific sites into many separate fragments. A centrifuge is a machine that uses a rotating container inside it in order to force all of the liquids to the bottom of the test tubes within…

Since obesity is on the continual rise, there has been a lot of research done to determine the best way to decrease weight. For many years, studies of body weight regulation have focused almost completely on caloric intake and energy expenditure.23 Since the obesity epidemic continues to skyrocket, one diet that has received a lot of attention is Intermittent fasting (IF), or periods of voluntary abstinence from food and drink.24 In some places, IF has been practiced since the earliest of…

pairs. Three common restriction enzymes: EcoRI, HindIII and PstI are used to separate the DNA fragments. The task at hand was to separate the fragments of DNA. Separation was to be done based upon size using gel electrophoresis. Then compare the DNA fragment of known sizes by millimeter or molecular weights, or standards to the DNA restriction fragments. Each enzyme cuts at a different place in the lambda DNA. This happens because each enzyme has a different restriction fragment that would…

industrial processes due to their natural metabolic capabilities are known as biotechnology. They are used a several ways such as manufacturing of foods, cosmetic products and production of antibiotics, probiotics, drugs, vaccines, insecticides, and enzymes. In addition, they are also being widely used in the genetic engineering. Nowadays, bacteria are commonly used in the food industry for the manufacture of dairy products such as cheese, milk and yogurt and also in the fermentation of alcohol.…

3122260 Date: October 15th, 2014 Introduction Metabolism consists of biochemical reactions catalyzing the conversion of complex macronutrients into usable energy by various enzymes. Energy in food is extracted from three macronutrients - carbohydrate, protein and fat. As food passes through the digestive tract, various enzymes and chemicals in the body break food particles into macronutrients and macronutrients. Macronutrients are the energy providers that are further processed into…

Topic: Molecular mechanism of dietary restriction in ageing Title: Dietary restriction and mTOR signalling pathway in lifespan extension Dietary restriction (DR), which is reduced intake of all dietary constituents except vitamins and minerals, was first shown to extend the lifespan of rats 80 years ago (McCay, 1930). Since then, many studies have proven that DR can extend longevity on wide range of taxa (Fontana et al., 2010). In addition, research done on animal models reveals that there…

Phenylalanine hydroxylase is responsible for converting Phe to Tyr and requires the cofactor tetrahydrobiopterin (BH4), molecular oxygen, and iron for its action (Blau et al., 2010). The reductant, BH4 is maintained in the reduced state by the NADH-dependent enzyme dihydropteridine reductase. Decreased PAH activity results in elevated Phe concentrations in blood and body tissue. As a result, approximately 75% of untreated patients experience severe neurological dysfunction (Brumm and Grant,…

Introduction(Title here) In the era of technology and overload with information, the right choices in health care are crucial. Medical professionals have earned high respect from public, and opinions of doctors and nurses about health-related matters play vital role in the community well-being. The topic of anti-aging medicine and strategies became noticeable popular in the last decade (Schweda & Marckmann, 2013). The public demands resources to improve, prolong or/and even reverse aging…

of catechol oxidase used from a solanum tuberosum specimen Abstract The various effects of temperature and pH on the enzyme activity and action of catechol oxidase, an enzyme containing copper which oxidizes phenols such as catechol with the use of dioxygen, were examined throughout several trial procedures. The experiment was conducted to determine the optimal pH for an enzyme to carry out its action, as well as to determine…

Chitin is the second most abundant natural polysaccharide consisting of β(1→ 4)-linked N-acetyl-D-glucosamine (GlcNAc) units in a linear form. Chitin is insoluble in water and mainly exists in two crystalline (α- and β-) forms. The α-chitin consists of sheets of tightly packed alternating parallel and antiparallel chains (1) and is abundant in the exoskeleton of arthropods, insects, fungi, and yeast cell walls. The chains are arranged in parallel orientation in β-chitin (2), which occurs less…