Restriction enzyme

Topic: Identifying Unknown Substances. Equipments: • 5 test tubes. • 1 beaker of Acid 1 beaker of water • 4-Litmus papers • 2 Pipette bulbs. • 1 robber stopper. • 1 Bunsen burner. • Q-tips(4). • Scoopula. Observation: Which substance is which? Hypothesis: All the unknown substances that we have are soluble in water. Procedure • We had a 4 test tubes filled with small amount of white powder. We labeled each test tube. For example the first test tube was labeled as “Substance A” , the second…

In this investigation, electrolyte content will be measured and compared to the sugar content to give a measurement of how drinks compare in terms of benefit during exercise. The independent variable will be the drink being tested, and the dependent variable will be the electrolyte and sugar content of that drink. Electrolytes are positively or negatively charged ions that are essential for survival. Positively charged electrolytes move towards a cathode, while negatively charged electrolytes…

pH will affect enzyme function by increasing the reaction rate because as temperature increase so is the rate of reaction and extremely high or low pH will result in the denatured by decreasing the reaction rate. The function of this experiment was to investigate the influence of the concentration of enzyme on the rate of reaction. The rate of reaction in this experiment was subjected by the amount of Guaiacol, H2O2, Extracts, and Buffer (pH5). The reaction rates with different enzyme…

〖Na〗^+and K^+channel relationship Figure one shows the temporal relationship between 〖Na〗^+ conductance (g_Na) and K^+ conductance (g_(K )), and the relative changes in the V_m during an action potential. 〖 g〗_Na increased as the V_m moved towards 0 mV causing depolarization of the membrane and started to decrease immediately after the peak of the action potential. Whereas, g_(K ) increased immediately following the action potential peak during the repolarization of the plasma membrane where…

Imagine trying to figure out one possible combination to a mixture, when there are over 100 possibilities. The 8th graders had to look at the physical and chemical properties, to somehow figure out the mixture. A group of students thinks that Baking Soda and Citric Acid is what’s in the mystery mixture. That group thinks that Baking Soda and Citric Acid is in it for a lot of reasons. One reason is that the mystery mixture has circular round objects in it and the Citric Acid is circular and…

various temperatures on Enzyme activity Introduction: The basic properties of life revolve around chemical reactions. Without the presence of enzymes some of life's processes would not happen easily. An enzyme is a macromolecule serving as a catalyst, they are generally a protein based molecule, as it is in the lab, Enzymes are organic catalysts that control the rate of chemical reactions in cells while not being permanently changed or without being consumed, which makes enzymes different…

Introduction Enzymes are an important biological catalyst that is absolutely crucial for life to exist. The ultimate purpose of this experiment was to examine enzymes and how they function in cells, explore the result that concentrations and environmental factors have over these functions, and lastly to read and interpret graphs related to enzyme function. This type of study had various parts, therefore there are various hypotheses. The first being, if it is potato extract, then it will contain…

Adenosine Triphosphate (ATP) is a nucleic acid present in organisms with the primary function of providing energy. It is comprised of three phosphate groups with one adenosine attached and releases energy by being hydrolysed into Adenosine Diphosphate (ADP), therefore one bond is broken and a phosphate molecule is lost. ATP can only be reproduced through phosphorylation which results in the addition of one phosphate to Adenosine Diphosphate. ATP must be regenerated in order to allow an organism…

Enzymes are biological molecules (proteins) that allow certain chemical reactions to take place much quicker than reactions would occur on their own. Enzymes function as catalyst, which means they speed up reactions by providing an alternative reaction pathway of lower activation energy (cite). Each step of a pathway typically requires a specific enzyme, without the specific enzyme, the pathway can not be completed. In addition, enzymes do not undergo permanent reactions and remain unchanged by…

Catalase containing, positive, bacteria consist of aerobic, oxygen respiring, and facultative anaerobic, can grow either with or without oxygen, bacteria. Being catalase positive means the bacteria is exposed to hydrogen peroxide in a concentration high enough to make it toxic to the bacteria cells. Thus, the cell needs to break down the hydrogen peroxide into water and oxygen gas to survive. Catalase positive bacteria can include beneficial bacteria. Two harmful bacteria that are catalase…