Restriction enzyme

Abstract: Using the PhysioEx.9.1 software, I ran Activity 4: Simulating Filtration lab to learn about the filtration of solutes in a membrane system. This experiment was conducted using four solutes: NaCl, urea, glucose, and powdered charcoal. The goal was to learn if any of these solutes passed through the three different dialysis membranes at varying pressures. Does increasing the pore size increase filtration rate? What effect will pressure have on the filtration rate? These are the…

glycolysis, using different tissues to compartmentalize opposing metabolic pathways. Uridine diphosphate (UDP) glucose is a nucleotide sugar used in many pathways. It is the precursor for glycogen and sucrose. It can also be used as enzymes to breakdown reactions. These enzymes are called UDP glucose pyrophosphorylase and UDP glucose dehydrogenase. The pathway that…

Create a table consisting of four columns that features: time intervals of 30 seconds, number of bubbles that have risen in the warm sugar water yeast mixture, number of bubbles that have risen in the cold sugar water yeast mixture, and the number of bubbles that have risen in the distilled warm water yeast mixture. Fill the given plastic container with warm water halfway. Place a thermometer in the plastic container, and record temperature. Acquire scale, position wax paper over platform, and…

experiment, the effect of environmental conditions on enzyme functions was tested. The materials needed for the experiment was an amylase enzyme to lower the activation energy of the reactions, starch solutions of pH 3,4,5,6,7,and 8, these provided different concentrations to project various environmental conditions. In addition, Iodine solution, and a spectrophotometer were needed. Each cuvette contained a specific pH concentration. Then the enzyme extract was added to each pH concentration…

In the science world, the term “catalyst” refers to something that speeds up a reaction. A catalyst increases the speed of a reaction without changing itself. Instead of asking why things happen, we should ask how things happen. What elements were involved in the equation and what catalysts (if any) were involved? In historical terms, asking how is far more valuable than asking why. For example, US citizens in 1963 may never be able to understand why President Kennedy was assassinated. They…

Chromatography is a key aspect in chemistry. It allows one to separate molecules based on a variety of things. In labs 3 and 4 we looked at two of the characteristics that molecules carry that can help separate them. Lab 3 used spectrophotometry once again, but the concept that garnered the most interest was ion exchange chromatography. Ion exchange chromatography works by separating molecules based on the individual charges they carry. In our case we used anion exchange chromatography, which…

Our body needs to have an equilibrium in order to function properly. The process that is known to stabilize and regulate our body to function properly is called homeostasis. The homeostatic mechanism has three components: the receptor, the control center and the effector. Receptors are one of the components that provide the information of the sudden fluctuation in the body, then they transmit a signal from the nerves to the control center. The control center processes that information and…

The purpose of this experiment is to examine the extraction of the DNA material that was extracted from the Chromosomal DNA from E coli in laboratory 9. DNase is used to help extract the Sugar phosphate backbone of the DNA. DNase should only cleave DNA not RNA but DNase can classify contaminated RNA from chromosomal DNA. Methods A. preparation :E. coli was used to extract the DNA as instructed in laboratory 9 to use in the experiment on analyzing in this experiment. There was 500 μL of the…

α-Lactalbumin, a multifunction protein in milk, forms an enzyme complex with galactosyltransferase that catalyzes the last reaction in lactose synthesis.1 α-Lactalbumin has one Ca2+ binding site and several Zn2+ binding sites as well as many intermediary folding states, making it ideal for research involving the effects of cation binding on protein interactions as well as research on protein misfolding.1 α-Lactalbumin gives milk an antimicrobial property, making it important for infant health,…

There are factors that can affect the enzymatic activity. Enzymes are known to speed up the biological reactions. It is specific to its substrate which in this experiment is known to be catechol. The catechol oxidase is used in all parts to express the concentration in the enzyme reacting into ortho-quinone (Schultz, 2006). The catechol oxidase if from the potato that was used in this experiment. The enzyme was extracted as the full strength after centrifugation and used the homogenization as…