Replication

Introduction Before researching about the possibility of using telomerase inhibitors to kill cancer cells, we first need to know what cancer is and what telomerase is. Cancer is the name used to describe the diseases in which cell have abnormal growth or cell divides is uncontrollable and it will affect other cells. These cancer can spread to other part of the body through the blood or lymph systems. Many people think that cancer is just a disease, in fact, cancer is not just a disease but it…

Kary Mullis was born December 28, 1944. Mullis worked as a DNA chemist for 7 years, He also invented the polymerase chain reaction. Kary Mullis was born in Lenoir, North Carolina. Mullis is 71 years old, He’s also been married 3 times. He wrote a quote stating “Art is subject to arbitrary fashion”. Kary Mullis accomplishments were very overall. His invention of the polymerase chain reaction chain reaction helps specific segment of DNA results quickly and accurately. Methods obtaining a…

same biological information. DNA analysis is important in genetic, forensic science and medical research. PCR (Polymerase Chain Reaction) device has become an important analytical equipment of molecular biology. It is an instrument that gene replication of the DNA polymerase in the specified temperature. PCR technology can be widely used in the…

The random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) method was used to examine the genetic variability of Aiolopus thalassinus (Fabr.) populations at heavy metal polluted and unpolluted locations of different geographical distances. Four primers yielded a total of 69 scorable bands, which are all polymorphic. Cluster and Principal component analysis separated the inside and outside Cairo populations into two main branches that were further separated into smaller ones,…

Electrophoresis Kendra Gibson November 17, 2015 A scientist drops DNA samples in small indentations at the end of a Jell-O-like gel. He turns on an electrical current at the other side of the gel. His anticipation grows as he watches the samples slowly move from one side to the other. He pulls out a ruler and makes some measurements. He steps back in surprise, for he had just identified the most feared serial killer in the world! He did this by using Gel Electrophoresis. Electrophoresis (the…

Basic Biology The documents I attached include a lab report, worksheets, notes and one exam. My most memorable experiences in biology were in the lab. I got to test the effectiveness of lactase drops when exposed to solutions containing different pH or temperatures. My results indicated that the lactase enzyme had the optimal catalytic activity at temperatures similar to those found in the human stomach; however, the low pH in the stomach–ranging from 2 to 5– rendered the drops ineffective…

In order to evaluate the efficiency of RAPD-PCR fingerprinting for Cajanus cajan L. cultivar, the DNA templates for 7 RAPD primers banding modality were used. The total number of magnified fragments in the Cajanus cajan L. cultivar value of each primer was represented in Table 2. Total of 18 bands were obtained for all primers. The size of the amplified bands was ranged between 1000 bp and 300 bp. Primers OP-A5, OP-B4, OP-B9, OP-C5 and OP-E15 showed at least three specific fragments. Primer…

Assignment 3.05 DNA DNA, deoxyribonucleic acid, is the stuff in humans and almost all other organisms. About every cell in you and everyone else's body has the same DNA. DNA is usually located in the cell nucleus (nuclear DNA),however small amounts of DNA can be found in the mitochondria (mitochondrial DNA or mtDNA). The DNA has information stored as a code created by four chemical bases: adenine (A), cytosine (C), guanine (G), and thymine (T). Each base is connected to a sugar molecule and…

I also prefer the term "melting", though I recognise that this term is potentially confusing for a lay or beginners' audience. The analogy with melting of water ice, while intuitive, may invite the misconception that DNA "dissolves" into very small molecules rather than separates into its two strands. The lede states "These thermal cycling steps are necessary to physically separate the strands (at high temperatures) in a DNA double helix (DNA melting)", and in the description of the PCR steps it…

In Procedure 6.1, two different gels were used. One gel was 2.0% agarose while the other gel was 0.8% agarose. The reason we poured two gels was because each gel had a different purpose. The 2.0% agarose (thicker gel) was used to determine amplicons while the 0.8% agarose (thinner gel) was used for restriction fragments. One gel was ‘thicker’ than the other gel to distinguish smaller segments than the thinner gel. By having a thicker gel, smaller segments can move better and not be cleared out…