Measurement of glutathione Glutathione (GSH) is a ubiquitous tripeptide which serves several vital functions including detoxifying electrophiles, scavenging free radicals and providing a reservoir for cysteine. The sulfhydryl group of GSH reduces 5-5'-dithiobis (2-nitrobenzoic acid) (DTNB) and form the yellow colour product 5-thio-2-nitrobenzoic acid (TNB)21. Measurement of the absorbance of TNB at 412 nm provides an accurate estimation of GSH in the sample. Figure 7. Materials 5×105 cells/mL…
The experiment made use of two reactions in synthesizing 1-phenylazo-2-naphtol, also known as Sudan-1. The first is the diazotization reaction in which a diazonium salt is formed from the conversion of primary aromatic amines, such as aniline, to compounds with the nitroso functional group using a nitrous acid, such as HNO2. The diazonium salt was then used as an electrophile in a coupling reaction with an aromatic compound, β-naphthol. Recrystallization was conducted and yielded 0.18 g of a…
Phase Diagram and Phase Transformation Fig. 1 shows (FCC) Nickel-(BCC) Chromium binary phase diagram. The eutectic reaction takes place at 1345°C and the composition is 51% Cr and 49% Ni. The solid solubility of Cr in Ni is extensive which is evident from the phase diagram. Phase transformations of two Ni-Cr alloys are explained below. One is Ni40Cr60 (Line 2) which is closer to eutectic composition and contains greater % Cr and the other alloy is Ni68Cr32 (Line 1) which contains greater % Ni.…
be stated as a chemical compound of Silicon and the carbon. Edward G. Acheson was running an experiment on the synthesis of diamonds. At that time, it was produced by a high temperature electro chemical reaction of sand and carbon. We can also find the silicon carbide very rarely in nature in very small amounts. Also Edward G. Acheson was the first person to synthesize Silicon carbide. He used to pass an electric current through a mixture of carbon and clay for the synthesis of SiC. Still today,…
Introduction Quartz is one of the most plentiful minerals on earth, and it is found in various igneous, metamorphic and sedimentary rocks. Clear Quartz is composed of pure silicon dioxide, and has nearly smooth sides with naturally faceted points at one or both ends. Clear Quartz is also known by the name Ice Crystal, which was derived from the Greek word “krystallos,” which means “icy cold.” (1) Greek philosopher’s believed that this crystal was water frozen so hard that it could never thaw.…
carried out in acidic conditions, the reaction yields a Markovnikov alcohol. The mechanism includes the formation of a carbocation intermediate, which has a lower activation energy when it is formed on the more substituted or resonance stabilized carbon. The nucleophile then attaches to the positive charge created by the carbocation, yielding the Markovnikov product. However, if the reaction is carried out via hydroboration (with diborane or…
MANUFACTURING COSTS: Functional Elements: The baby stroller is mainly composed of three materials: Nylon (woven, as fabric) Plastic (injection molded, for plastic parts) Steel (tubing, for frame) Fasteners are included in the assembly of different components of the strollers. The stroller would have to be manufactured on an assembly line. The fabric weaving and seat making, the plastic molding and the frame can simultaneously be manufactured in their respective work areas. Following are…
compelling force of nature once more; Jute bags. Natural fibers s will assume a key part in the developing "green" economy in light of energy efficiency, the utilization of renewable encourage stocks in polymer items, modern procedures that decrease carbon discharges and recyclable materials that limit waste. Natural fibres are a sustainable asset, second to none – they have been revived by nature and human ingenuity for…
1.Bi Metallic bearings Steelair’s bimetallic bearing represents advancement in bearing technology and it can be assembled, accustomed or soldered into place. Along with high load capacity and good fatigue properties, it is also an excellent wear-resistant material. The bimetallic bearing is the composite of low friction copper alloy and is perfect for use when provided with excellent requisite strength and the finest carrying capacity by means of derived remains and secondary wedging technology.…
Carbohydrates have the following basic composition. They are molecular compounds made from three elements: carbon, hydrogen and oxygen. Monosaccharides are simple sugars with multiple OH groups. Based on number of carbons 3, 4, 5, 6, a monosaccharide is a triose, tetrose, pentose or hexose respectively. Disaccharides are condensation products of two monosaccharide units; examples are maltose and sucrose. A disaccharide is 2 monosaccharides covalently linked molecule. Polysaccharides are polymers…