Reference electrode

Electrophoresis; Separation of Proteins Purpose: To create an electrophoresis gel in order to further separate Cytochrome C, Myoglobin, Albumin and Hemoglobin(proteins) based off of their net charges. Materials: • Rabbit hemoglobin • Serum albumin(cow) • Myoglobin • Cytochrome C • Microtubes • Tape • Micropipets • Agarose gel • Buffer • Gel chamber • Test tubes • Test tube holders • Gloves • Macropipets Properties of Proteins: Protein Color Isoelectric Point Net Change at pH 8.6 Cytochrome C…

Reference was also made to deception being required to keep the experiment legitimate by placing the test subjects in the situation rather than simply asking them to hypothesize. Further to this, Forward, Canter and Kirsh (1976) state that deception is an…

In this study, we developed an anthracene-based fluorescence PET sensor (AMG) was developed for detecting high pH values and water content in organic solvents. A guanidine group as a proton receptor was covalently attached to an anthracene fluorophore to afford highly polar AMG, with reasonable solubility in highly basic solution. The fluorescence of AMG increased almost linearly with decreasing pH in the range 11.0–13.0. AMG also exhibited a fluorescence turn-on response to water in various…

Fat is a Drosophila tumour suppressor gene that regulates the growth of cells in tissues. The McNeill lab are trying to understand how Fat restricts growth and controls Hippo pathway activity. This is being tested using flies and mice. They are also investigating how Fat regulates growth via control of metabolism and mitochondrial function. The McNeill lab is hoping to find how Fat regulates the growth rate and full grown size of organs. Fat also controls a high-level form of tissue…

Figure (2.6) shows industrial oxygen sensor. Fig. (2.6) Oxygen sensor [25] This thesis used some kinds of sensors described below: a. pH Sensor “The term pH is derived from a combination of “p” for the word power and “H” for the symbol of the element Hydrogen. Together the meaning is the power of hydrogen. pH used to compare the relative acidity or alkalinity of a solution at a given temperature. A pH of (7.00) describes a neutral solution because the activities of hydrogen and hydroxide…

added as well as a buffer; KH2PO4, 15 ml of H20, and 10 ml 0.1 M HCl which were all measured by a graduated cylinder into each bottle. The pH was then measured and recorded in bottle number one. The next two bottles were buffer solutions and the pH electrode was placed in bottle number two while 0.5 M NaOH was added from the buret while mixing until the reading stabilized to a target pH range. This was then done to all bottles. The spec 20 was then used to measure the absorptions of the four…

sample) • Buffers 2,4,5,7. Method: • Calibration of the pH meter with buffers 2, 4 and 7 washing the pH electrode between each measurement, and reading with pH 5. This was recorded and printed on a weight receipt, and the slope of the measurement had to be within specifications to pass. • The pH electrode was then inserted into the top sample of stiprox 1.5, and the pH was read, and the pH electrode was washed, this was repeated for the three other samples. • The results obtained were then…

that if the animal was defibrillated shortly after going into fibrillation, there was a chance it could recover. In 1947, when he first used this method to revive the 14 year old boy, he used the very first defibrillator, which used spoons as the electrode…

Kate Byrne DT299/4 CHEM 4128 Applied Physical & Analytical Chemistry Assignment Liquid Chromatography High Resolution Mass Spectrometry Hyphenated Techniques The term “hyphenated technique” was introduced by Hirschfeld in 1980. It refers to the combination of a chromatographic separation technique and a spectroscopic detection technique to form a new, more efficent instrument. The technique which will be discussed in this case is LC-MS. The aim of combining both techniques is to purify and…

Measurements of Stress in Life Science Stress can be defined as the physiological, psychological and behavioural responses towards change. It is a common problem of humans’ and it is increasing progressively. Conditions of stress are work load, work place, conflicts between co-workers, social life issues, environment and time pressure. It has two types: eustress or positive stress which means performance improvement and distress or negative stress which can lead to mental and physical…