Part A: Background Information Acids and bases are very common in today’s society, and they can be found in most households. Acids can be found as by-products of air pollution, concrete cleaner, food flavouring and is produced by the muscle. Bases are found in most cleaning products as ammonia or sodium hydroxide, and they also treat indigestion. There are many ways that acids and bases can be defined by their chemical properties. The Bronsted-Lowry definition states that acids are proton…
Pure Copper Iodide were synthesised and the mass percentage of Copper in purified Copper (I) Iodide sample were determined by applying Beer’s Law in UV-Vis spectroscopy. Absorbance value of Cu-cuprizone solution was measured. Using the absorbance value of 0.3576 , the concentration of the Cu-cuprizone solution were obtained at 0.001389g/L with reference to the graph of the standard solution A to D. The graph of absorbance against concentration of the standard solution A to D shows a linear…
Objective The purpose of the experiment is to synthesize multiple cobalt complexes with various ligands and explain the ligand field transitions of these complexes using Ultraviolet-Visible (UV-Vis) spectroscopy. The experiment provides insight into specific colors of certain molecular species and respective bonding information. Introduction Transition elements are defined by the IUPAC as atoms that have an incomplete d-subshell, most commonly referring to the d-block.1 When transition metals…
Biochemical foundation for each of the four enzymatic assays performed on each cellular fraction. We performed various assays on each of the four log phase culture fractions, as well as the extracellular fraction from a stationary phase culture, to test the localization of the enzymes. For the protease assay, a specific soluble chromogenic substrate called azocasein was employed to determine presence of enzyme activity. The degradation of this substrate in trichloroacetic acid produces a colour…
A small burette funnel was filled with column packing solvent at mobile phase, high boiling petroleum, in this case hexane (and then 10%acetone hexane mixture), and Alumina as stationary phase. When adding the sand and the alumina they have to be first mixed with the hexanes in order not to disturb the column. The Lycopene is Red- Orange and more attracted to alumina due to its high degree of Unsaturation, which can be expected to be eluted after Yellow-Orange Carotene pigments. And the other…