Reagent

Carbonyl compounds are the compounds the contain C=O group which is called carbonyl group (double bond oxygen to a carbon). There are many types of carbonyl compounds such as aldehydes, ketones, carboxylic acids, esters, amides etc. Aldehydes and ketones are the oxidation products of alcohols. Depending on the alcohol’s structure, the product obtained will be different. Primary alcohols have one carbon and two hydrogen atoms attached will produce aldehyde and then to carboxylic acid in two…

failed to react with anti-D antisera at immediate spin. An indirect antiglobulin test is essential to identify patients with the Weak D antigen. The red blood cells are incubated at 37˚C with anti-D and then tested with anti-human globulin (AHG) reagent. This test is done on prenatal patients and candidates for Rhesus immune globulin. Du is also done on Rhesus negative patients to ensure they are truly Rhesus negative. It may or may not be done routinely on Rh negative candidates for…

The purpose of the unknown experiment is to understand and identify a gram positive and a gram negative. Bacteria can be very helpful in clinical uses and many pharmaceutical drugs are based on the organism. Knowing how a bacterium works and how it is structured can be crucial to determine how it affects each individual. Several biochemical tests were performing to separate and determine the identities of the microorganisms. Broth tube number 31 containing unknown microorganisms. One of the…

Taylorann Smith Abstract The purpose of this experiment was to find and compare the amount of protein in Chobani Greek yogurt and Yoplait yogurt, to the standards given by the lab instructor. The two samples were diluted and added to the Bradford Reagent, which allowed for the absorbance of each sample to be found. The hypothesis was that, because Chobani Greek yogurt is thicker than Yoplait yogurt, it would have much more protein in it. The results showed that there wasn’t a big difference in…

water was placed in a test tube. A few drops of the of the unknown was added to the test tube and mixed with a pipette. After a few minutes, the unknown was determined to be insoluble. Next, the Schiff’s test was conducted by adding 2 mL of Schiff’s reagent to a clean and dry test tube. Also, 4 drops of the unknown were added to the test tube and mixed with a pipette. The Schiff’s test was determined to be negative. Then, the Bisulfite test was conducted by adding 1 mL of Bisulfite solution to a…

second sets of samples were extracted during the fasting state. Water was then added to a beaker and it was set on top of the hot plate. After the water was boiling, Benedict's reagent was placed in to the six test tubes up to two centimeters. The first color of Benedict's reagent was blue. As glucose levels rise, Benedict’s reagent changes in color gradually from blue to yellow to orange to red. The first three test tubes of the blood vessel samples taken from postprandial were placed in the…

Preparation of polyallyl polyether of allyl sucrose Abstract: Allyl sucrose is prepared by using sucrose, allyl chloride, NaOH and DMSO. This novel method is less time consuming and required less solvent than other methods. The product is characterized by Fourier Transform Infrared (FT – IR), 1H-NMR and MASS Spectrometer. Key words: allyl sucrose, allyl chloride, sucrose Introduction: Allyl sucrose is used for…

reaction. Likewise, Borneol should be the minor product in the reduction of Camphor. As such, it is likely that less of it was formed during the actual reaction. The experiment began with 0.250 grams of Camphor, and since Camphor is also the limiting reagent, 0.250 grams of the final product should be obtained. In the experiment, 0.212 grams of the product was obtained leading to a percent yield of 84.8%. Some reasons why the percent yield was not 100 percent could include some of the…

Staphylococcus epidermidis is a gram-positive cocci, that measured approximately 0.5um and had an optimum growth temperature of 37°C. Generally, when given an unknown solution, Staphylococcus epidermidis has features that can distinguish it from other forms of bacteria. After doing a gram stain, it was noticed that the bacteria had a cocci morphology, meaning that the cell can either be staphylococcus, enterococcus, or micrococcus. Using the gram stain, it can also be seen that the bacteria in…

Reagents 2,4-dichlorophenoxyacetic acid (2,4-D) (Sigma-Aldrich), 2,4 dichlorophenol (2,4-DCP) (Sigma-Aldrich), ferric sulfate (nH2O) (Carlo Erba), hydrogen peroxide (H2O2) (Carlo Erba), acetonitrile (Sigma-Aldrich) and Milli-Q water were used to perform all the experiments . All the reagents were used without further purification. Natural groundwater samples Samples were taken from groundwater wells localized in a small rural community (coordinates: 3º21’03.38” N, 76º26’24.51”) near to the…