Reagent

vegetables are simpler form than found in roots or leaves. Most common assay being used to determine the total phenolic content by using Folin reagent. This reagent is not detecting the exact phenolic groups contained in the extract. Total phenolic content in this assay is determined by measuring the discoloration of blue colored complex alkaline of Folin reagent using a spectrophotometer and the reading is expressed as gallic acid…

There were many tests performed on my unknown, which led me to conclude that the unknown microorganism is Pseudomonas fluorescens. When determining the gram reaction of the unknown, a Gram stain was done. This process differentiates between a Gram-positive and Gram-negative microorganism. This is done in four steps. The microorganism was stained with iodine, crystal violet, alcohol and acetone, and safranin. Iodine was used first as it acts as a mordant. It forms an insoluble complex by binding…

P. putida and P. fluorescens isolates were cultured in 250 ml Laurea Broth (LB) medium at 30±1 ºC for 36 h in an incubator shaker at 150 rpm. The culture was then poured into 50 ml falcon tubes and centrifuged for 10 min. at 6000 ×g at 4 ºC. The pellet was washed thrice in 15 ml distilled water and centrifuged. Then it was dissolved in distilled water and the optical density (O.D) of the culture was set at position-1 at 600 nm=107–108 colony forming units (CFU) ml-1 for inoculation (Bhuvaneswari…

Results/Discussion: 2-butanol and Acetic Acid were refluxed together with anti-bumping granules and a catalyst of sulphuric acid for 1 hour and five minutes. Anti-bumping granules are small ceramic granules which have rough edges. They are added within a mixture to encourage smooth boiling. In this experiment by adding the anti-bumping granules we were able to achieve smooth boiling throughout both the reflux process and the distillation process. Without adding these granules, bubbles of vapour…

The purpose of this experiment is to learn how to prepare albumin from egg white and to understand the basics of protein fractionation using ammonium sulfate. Ovalbumin is the major protein of egg white and is fractionated by means of ammonium sulfate precipitation. The properties of the solvent system will dissolve the protein and greatly impact solubility. In total, there are four major factors that affect protein solubility: ionic strength, pH, temperature, and dielectric constant of solvents…

Laboratory Analysis Justyna Sudyka 1. a. Clostridium sporogenes grew throughout all of the thioglycollate broth, but there was more growth towards the bottom. The aerobic TSA + glucose plate did not have any growth, but the anaerobic plate did have growth. Escherichia coli grew throughout all of the thioglycollate broth as well, but the organism was clustered slightly more towards the top. There was growth in both aerobic and anaerobic TSA + glucose plates. Micrococcus luteus only grew at the…

Microbiology Technician, Kent A world-leading provider of diagnostic products to the science industry is growing and require an experienced microbiology technician to join their Immuno- microbiology team. You will be responsible for the production and processing of polyclonal antibodies for streptococcal, staphylococcal and other assigned latex agglutination products. You will have at least a year in industry demonstrating core microbiology skills. Job Purpose To operate as a Technician…

used instead. The bottom-layer (dichloromethane and sample) was run through the Rotavap® and the sample residue that was left was reacted with 1-pyrenehexanoic acid. The second step of forming the amide also required TLC, and was done using the same reagents, ethyl acetate and water. The spots, however, on the plate could not be differentiated visually, and thus the reaction may not have run to completion. Mass spectrometry was used instead, and it was determined that the reaction had failed by…

Assignment: Chemistry of Magnesium and Silicon Magnesium Properties Magnesium is a group is a group 2 element with the symbol, Mg, an atomic number of 12 and has a molar mass of 24.31 [1]. It is made up of 12 protons, 12 neutrons, and 12 electrons. It’s electron configuration is 1s2,2s2,2p6,3s2 this given as noble gas configuration is [Ne]3s2. It has a melting point of 650 °C and a boiling point of 1090 °C [2]. It has a metallic radius of 150 pm, a coordination number of 72(6), and a density of…

solution underwent a single replacement that yielded magnesium chloride and hydrogen gas. Additionally, the ideal gas law was used in this experiment to find the moles of hydrogen gas. Another topic that relate to this lab was the limiting reagent. The limiting reagent in this experiment was the magnesium. Additionally, the water vapor pressure was present during the experiment, which was used in the calculations. Another topic was the signs of a chemical reaction. The sign that a chemical…