Abstract As one of the most popular analgesic and antipyretic, especially for pregnant women and children, acetaminophen is available over the counter in many forms. When consumed over long periods of time or in high dosages, acetaminophen has serious side effects including liver damage and other organ damages, therefore an effective quality control method of analysis. Here a method using cyclic voltammetry to analyze the concentration of acetaminophen in a liquid suspension Publix brand…
Literature Review: Green Fluorescent Protein and Histidine Tagged Proteins Histidine and green fluorescent protein (GFP) tagging are important in molecular biology because they allow for purification, tracking, and quantification of target proteins (Ferrari et al., 2004; Cho et al., 2011; Deponte, 2012). Histidine tagging provides a method for isolating and increasing the amount of target protein recovered from a biological organism or mixed sample of proteins (Masek et al., 2011; Singh and…
The International Association for the Study of Pain (IASP) defines human pain as an unpleasant sensory and emotional experience associated with actual or potential tissue damage, or described in terms of such damage (Loeser, 2008). It is a complex sensory experience that can even be modifiable without the use of a physical harm. While in contrast, there isn’t an animal-based definition of animal pain. A commonly used definition for animals is ‘ an aversive sensory experience caused by actual…
life, we decided to investigate the experiment about metabolic rate caterpillar, Manduca sexta. There are many techniques used to measuring the metabolic rate, but there are only best method that can be done in laboratory: “quantification of heat production and quantification of gas consumption” (Perkin, 2015).Our investigation is about measuring the oxygen consumption of the caterpillar in the trial duration when it was placed in sealed chamber along with chemical absorbing CO2 produced.…
a viable method to achieve standardization and quality control. However, the research on the quantification of multifarious constituents in complex extracts from Phlomis species has not been developed. Recently, the chemical constituents of P. umbrosa have been investigated for their interesting and significant bioactivities. In this study, we describe the isolation, structural elucidation, quantification analysis, and anticancer and AChE activities of the isolated compounds from P.…
Twenty Korean barley cultivars (Geongang, Gwangan, Nackyoung, Dahyang, Daejin, Saegang, Boanchal, Keunal, Saessal, Saechalssal, Sujung, and Boanchalssal) were selected in this research. All the cultivars were developed by the National Institute of Crop Science (NICS), Rural Development Administration (RDA), Korea and were grown during 2013 in the experimental fields of the NICS, Milyang, Gyeongnam. After harvesting, barley leaves were immediately washed with clean sterile water and then…
oral administration; Cmax, the maximum plasma concentration; CV, coefficient of variation; HPLC, high-performance liquid chromatography; ka, the absorption rate constant; IS, internal standard; LLE, liquid–liquid extraction; LLOQ, lower limit of quantification; MRM, multiple reaction monitoring; PK, pharmacokinetic; QC, quality control; t1/2, the terminal half time, Tmax, the time to reach Cmax, UPLC-MS/MS, ultra-performance liquid chromatography-tandem…
Critical assessment of the article: Quantitative analysis by Auger Electron Spectroscopy 1 Introduction When the outer electron fills the vacancy left by an ionized electron in the inner shell, the released energy can be passed to another outer electron that causes further emitting of an electron with characteristic energy, this is the basic principle of the Auger electron spectroscopy (AES) [1]. Nowadays, AES is almost an indispensable tool in nanoscience research for its high surface…
lack of sensitivity hindered the application of LC-UV on the pharmacokinetic study of risperidone. Furthermore, requirement of large amounts of samples and long analysis time also restricted its application. LC-ECD was another choice for the quantification of risperidone [20-21]. But there were many practical issues, such as long time for stabilizing the detector, susceptible to be interfered by endogenous compounds, and restriction of mobile phases, and so on. LC–MS and LC–MS-MS methods have…
for research assistant is to learn the research skills towards a research career in MRI. I believe, this opportunity will harness the understanding of MRI, learn methodological programming skills in developing MR pulse sequencing and tools for quantification. This is an admirable opportunity to work, learn, and contribute at Sir Peter Mansfield Imaging centre. With everlasting interest in MRI, enthusiasm to learn, dedication to work and motivation to contribute; given this opportunity I aim to…