Punctuated equilibrium

6. Quantitative determination of rat peroxisome proliferators activators γ (PPAR-γ): Rat PPAR-γ concentration was determined by ELISA; using a commercially available kit, MyBioSource, Inc., California, USA. The kit utilizing a monoclonal anti- PPAR-γ antibody and an PPAR-γ-HRP conjugate enzyme-linked immunosorbent process assay (ELISA) to determine the concentration of PPAR-γ in serum, plasma, urine, cell culture supernatant and tissue homogenates. Principle of the assay: The sample and buffer…

The Michaelis constant, Km, is the amount of substrate needed for the enzyme to obtain half of its maximum rate of reaction. (Oregon 2015) It reveals the enzymes affinity for the substrate and shows the magnitude of the enzymes affect on changing the rate of the reaction. For example, an enzyme with a high Km needs a large concentration of substrate to efficiently increase the rate of the reaction. (Oregon 2015) A low Km indicates that the enzyme will increase the rate of reaction with a small…

few years later that a solution was proposed as to how rationale players would interact. John Nash proposed that players would adjusted their strategies until no player could benefit from changing and reach what has been coined the “Nash Equilibrium.” The equilibrium will leave the players at their best possible response to all other players responses (Camerer, 2003; University…

when playing the game repeatedly, cooperation is improbable in the Prisoner’s Dilemma due to backwards inductive reasoning. The Stag Hunt Game, on the other hand, allows cooperation to be far more stable because when both players cooperate, a Nash equilibrium occurs, so neither player would do better by changing their strategy. Thus, there would be no logic in withdrawing cooperation in the Stag Hunt Game, as this would only serve to harm both players. The Prisoner’s Dilemma proves to provide a…

Introduction An enzyme is a protein that functions as a catalyst, it speeds up chemical reactions (Freeman et al. 2017 p. 90). Enzymes are often large globular proteins and are able to hold substrates in specific orientations so they are able to react, the location where the substrate binds with the enzyme and reacts is called the active site and is the location where the catalysis occurs (Freeman et al. 2017). Enzymes activity is often related to the optimal environment for them in respect to…

shows a tendency to move towards the predicted level. In the case of R = ∞ (perfect discrimination), the level of dissipation fluctuated around the predicted dissipation level. Further, the average earnings were significantly closer to the Nash equilibrium under perfect discrimination. Thus, the authors concluded that the rent-seeking model has predictive power. In both cases, rent dissipation is observed and is in line with the theoretical predictions. The experiment also revealed three types…

Reactions: Chemical reactions involving aqueous species and mineral species are heterogeneous and slower compared to aqueous species reactions, thus modeled as rate dependent chemical reactions. This means that when mineral species (N_m ) are not is equilibrium with the aqueous species, the minerals can either dissolve or precipitate. As shown in equation R11 of an example of calcite dissolution/precipitation, a mineral species (Calcite) is involved in a reaction with only aqueous species…

Abstract: In this lab, three variables were tested to discover their effect on the enzyme catalase, taken from a cut and blended potato. The blended potato mixture was strained and put into a centrifuge, the liquid that was in the centrifuge contained the enzyme and remained on ice. The enzyme was placed in test tubes that had varying levels of pH buffers, substrate concentrations and temperatures; the pH, substrate concentration and temperature were the three variables. The data showed that…

different warfarin derivatives; this was to be determined by comparing the equilibrium constants (Keq) and the Gibbs’ free energies of a number of warfarin derivatives. To study this, four warfarin derivatives were used, namely racemate mixtures of 2’-Fluoro warfarin (2’FW), 3’-Fluoro warfarin (3’FW), (R)-4’-Fluorowarfarin,…

Enzymes are protein molecules that occur in all living things on earth and enable chemical reactions. Enzymes not only provide an alternate pathway for a chemical reaction, with a lower activation energy, they also bring substrates together in an optimal orientation, thereby speeding up the rate of reaction (Slide Share 2016). In an enzyme catalyzed reaction, the substrate (reactant) binds to the active site and forms and intermediate substance known as the enzyme-substrate complex with the…