Pseudomonas aeruginosa

Extraction was performed by using Soxhlet apparatus with 95% (v/v) ethanol. The dried extract was dissolved in Dimethyl Sulfoxide (DMSO) and used to assay the antibacterial activity against Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853. An ointment was prepared from the ethanol extract (10% w/w) and assessed for its in vivo wound healing potential on infected rat model by rate of healing, bacterial count, biochemical analysis, and expression of matrix metalloproteinase…

For this lab, the task given was to identify an unknown bacterium of the student’s choosing using Bergey’s Manual, unknown #6 was selected for this lab. Bergey’s Manual is a valuable resource because it provides classification based on rRNA when identifying an unknown bacterium. The purpose of identifying bacteria is for pragmatic purposes- like determining the appropriate treatment for certain infections and diseases. Through Bergey’s Manual, one should be able to successfully identify an…

were checked in various oils such as olive, coconut, sunflower and rice bran oils, the emulsification activity of biosurfactants. Pseudomonas aeruginosa had higher emulsification activity than Saccharomyces cerevisiae. While checking the sugar and lipid content in biosurfactants by phenol sulphuric acid method and vanillin reagent method, found high in Pseudomonas aeruginosa than Saccharomyces cerevisiae. Under this study, after centrigugation of crude extract,the pellets were suspended in a 40…

The bacteria used in the experiment are Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumoniae, and Salmonella pneumonia. These all bacteria will be culture in the nutrient broth. The first stage is making Nutrient Broth. Weigh out 5.8 grams of nutrient broth powder. Dissolve completely the nutrient broth powder in beaker containing 200 ml of distilled or the deionise water. Then the mixture is transfers to the vials. Those vials are autoclave and sterilise at 1…

Antibiotics are commonly used to treat chronic respiratory tract infections and prevent them. By the time most CF patients reach adulthood, many are colonized with mucoid producing strains of Pseudomonas aeruginosa.5 Numerous patients may also have chronic infections with Staphylococcus aureus. Quarterly culture and sensitivity results can be essential in directing acute antibiotic therapy.5 Antibiotics can be aerosolized (inhaled), taken orally, or…

On October 31, 2017, at 4:00 pm, I attended the Department of Biology Seminar Series hosted by Dr. Richard Gomer that helped in the Biological Sciences Building East, Room 115. The lecturer was Dr. Carolyn Cannon from the Department of Microbial Pathogenesis and Immunology at the College of Medicine TAMHSC. What intrigued me to go to this lecture was the title, “Wolves in Sheep's Clothing: Killing Bacterial Pathogens with Innocuous Compounds. Surprisingly the title did match the content that Dr.…

According to the Infectious Diseases Society of America (IDSA), antibiotic-resistant bacteria are becoming an increased problem in hospitals and communities due to the lack of antibacterial options currently available. Throughout the world, many Gram-positive and Gram-negative pathogens are growing more resistant to antibiotics currently on the market. The goal of this journal article is to raise awareness for the global antibiotic crisis and to hopefully encourage the community and legislative…

slightly more towards the top. There was growth in both aerobic and anaerobic TSA + glucose plates. Micrococcus luteus only grew at the top of the thioglycollate broth and it grew in the aerobic TSA + glucose plate but not on the anaerobic plate. Pseudomonas aeruginosa grew towards the top of the thioglycollate broth, but slightly below the section in direct contact with the air. There…

2000 by the Oklahoma State Department of Health Study linked 2 nurses, one with long natural nails and one with artificial nails, to an outbreak of Pseudomonas aeruginosa, a gram negative bacteria that can cause blood poisoning. They found that “46 neonatal intensive care unit patients in a university-affiliated children's hospital acquired P. aeruginosa and 16 of them died” (Moolenaar et al.,…

central component in regulating the immunological and physical barriers of the airway epithelium is Ca2+. The participation of Ca2+ as a second messenger is vital to numerous physiological processes The common respiratory bacterial pathogens, Pseudomonas aeruginosa and Staphylococcus aureus, activate 100 nM Ca2+ fluxes immediately upon contact with airway epithelial cells, and this Ca2+ signal initiates the activation of proinflammatory signaling events…