Pseudomonas aeruginosa

and 5.12 x 108 cfu/mg soil on MacConkey (MCC ) agar, indicating that the bacterial count is higher on MCC than on EMB. The bacterial isolates and their frequency of isolation from dumpsites were Escherichia coli (33.3%), Klebsiella spp.(16.7%) ,Pseudomonas spp. (13.3%), Proteus spp.(10%), Streptococcus spp. (8.3%), Salmonella spp. (6.7%), Staphylococcus aureus (6.7%) and Enterobacter spp. (5.0%). The high presence of pathogenic bacteria from dumpsites is of serious public health concern and…

produce acid, but no gas production was suggested from the dichotomous key. The test result for SIM deep slant that was used to test for sulfur-reducing turned up positive due to its color changed into a blackening color. The test result eliminated P. aeruginosa and identified unknown #63 as being Citrobacter freundii. During the unknown lab project, I was quite thankful that there were no mishaps in my lab work. There were no contamination, spills or accidents. The only problem I had…

INTRODUCTION Microbes are those microscopic organisms that can cause diseases in the human body. These microbes migrate with locomotive organelles called FLAGELLA, a very vigorous nanomotor that is highly conserved across bacterial species. Flagella allow the microbes to move towards favourable environments. Further studies on flagella has revealed that it not only serves the purpose of locomotion but also plays a very crucial role in bacterial pathogenicity(14). A flagella has mainly three…

Septic arthritis, also known as infectious or bacterial arthritis, is described as a painful infection that is present in a joint capsule in the human body. Synovial fluid, a lubricating substance found inside a joint capsule, is sterile in an uninfected joint. When a joint becomes infected, this synovial fluid is taken over by microorganisms that set up the infection. It is an uncommon infection, but it involves immediate treatment if detected due to its ability to permanently damage the joint.…

behind the eardrum which allows the eardrum to vibrate). It also shows the mastoid disease. The website shows pictures of outer ear and ear canal common problems such as acute otitis externa (an acute bacterial infection usually caused by Pseudomonas aeruginosa), ear tick (a tick imbedded in the ear canal that should be removed as soon as it is diagnosed),…

Acinetobacter baumannii is a gram negative coccobacilli bacteria. It is an aerobic Glucose non-fermentative and oxidase negative. This bacterium is able to avoid desiccation for more than 30 days. It can be Opportunistic pathogen in human and It is commonly isolated from the hospital environment and hospitalized patients causing nosocomial infection [1]. This bacterium is responsible for causing several diseases in human such as pneumonia, urinary tract infection, Meningitis, wound infection,…

3.3.3: Phytochemical analysis The leaves and bark extracts were tested for some secondary metabolites like-saponins, tannins, alkaloids, anthraquinones, phlobatannins, flavonoids, terpenoids, reducing sugar and poly phenols. Test for reducing sugar: Mixture of 1ml or 1gm of plant sample is taken in a test tube and 10ml deionized water is added in it after this a few drops Fehling solution (1ml Fehling solution A and B) is also mixed and heated at 1000C in a water bath. The Brick red…

Efflux is a method of facilitated transport carried out by protein complexes that fall into one of six currently identified classes based on their protein structure.44 These complexes are commonly able to recognize more than one substrate, making them a major cause of multidrug resistance, especially in Gram negative organisms.45 As many of these proteins are not expressed constitutively, it may be hard to predict the effect these complexes have on in-vivo treatment; many are tightly regulated…

2. Synthetic surfactants used in pharmaceuticals and their properties. 2.1 Classification of surface active agents. The widely used synthethic surfactants in pharmaceuticals are classifieds as according on their surface charges. 1.Anionic surfactants:sodium alkyl sulfonates .SDS,SLS. 2.Cationic surfactants:Quaternary ammonium chlorides (quats),pyridinium salts . 3.Zwetterionic (amphoteric) surfactants:phospholipids- Phosphatidylcholine (Lecithin). 4.Nonionic surfactants: spans( esters of…

membrane. Targeting of the bacterial cell membrane by polymyxins is specific for gram negative bacteria due to their specificity for lipopolysaccharide which is common in gram negative bacteria. Polymyxins are used to treat MDR strains of Pseudomonas aeruginosa. Polymyxins (which feature a cyclic peptide with a long hydrophobic tail) work by disrupting the structure of the bacterial cell membrane via their interaction with the cell membrane’s…