Protein

Protein Purification III Parts 09/15/2015, 09/22/2015, 09/29/2015 Kaisha Butz Lab Partner: Ryan Van Abstract The lab was comprised of three parts, the purpose of which was to separate a protein mix and analyze the results. The first part was separating the proteins; the second part was reacting the proteins with an enzyme, and the third part was analyzing each fraction from Part I to see if separation was successful. It was hypothesized that out of the four proteins…

III. DISCUSSION 3.1 retention of Proteins The results of statistical tests show that the addition of probiotic plus herbs on different feed real (p < 0.05) against retention of Red tilapia fish protein. Results the average protein retention indicates that P1 (4,7659%) differed markedly with P0 (1,1505%) but no real different with P1 P2 (3,3455%), P3 (4,1090%) and P4 (2,1154%). The treatment gives the same results Q4 low with P0 feed without granting probiotics plus herbs. Administering…

Protein mutations are often introduced at the DNA level in the corresponding gene, which takes advantage of the central dogma of biology (DNA is transcribed to mRNA; mRNA is translated to proteins. The introduction of mutations in target genes has become trivial with the advances in synthetic biology. Major classes of library construction include (1) random mutagenesis, (2) recombination, (3) semi-rational design, and (4) scanning mutagenesis. Together, these classes of library construction…

The Enzyme Electrophoretic Separation of Proteins lab experiment will take place on March 7th, 2017, from 2:00pm to 5:00pm. It is important for students to follow lab safety guidelines in order to prevent any complications that may be a risk to one's health. For this experiment, students must wear their lab coat, goggles, and gloves at all times. All materials in this experiment should be handled with caution to avoid any damages. Students must keep hands away from face, eyes, and mouth, when…

The genetic combinations; two normal genes, one normal and one cystic fibrosis, and two cystic fibrosis, each affect the membrane proteins involved in transporting chloride ions through the cell membrane. Two normal genes would not alter whether or not the membrane proteins would correctly shield the chloride ions so that the ions could pass through the cell membrane. With this ability of the chloride ions successfully transporting across the cell membrane the body would not generate a…

Proteins are a multifarious group of macromolecules, which carry out many different functions within the human body. (Mbuthia, 2009). They are comprised of repeating units of amino acids held together by peptide bonds. As stated by Mbuthia, the Biuret reagent is a solution which aids scientists to conclude if there are any peptide bonds present within a sample, and thus, is used to determine protein existence within a substance. The Biuret assays depends on the presence of 2 or more peptide…

Analysis of Consumer Products with Unknown Protein Concentrations Using Spectrophotometry Abstract Consumer products typically give detailed information about their ingredients and nutrients. However, the concentration of a specific nutrient may not always be clear from just the label information alone, and in some cases the product labels may not be accurate. Thus, it may be hard to compare the concentration level of a certain nutrient, such as protein, amongst different products. In this…

month of September an article was published in an ‘Emergency Nurse’ journal. The heading of this article is “Protein Test May Ensure Quick Diagnosis of Heart Attack.” We all know that the odds of knowing when a heart attack may happen are very low. While a group of research nurses have come to the conclusion that you may be able to predict when a heart attack may happen by doing a simple protein test. Heart attacks can be fatal and hard to detect. This is what makes this discovery and hypothesis…

Determining the concentrations of proteins is necessary for a variety of different reasons. For example, protein concentration must be known in order for proteins to be used in enzyme reactions, as the correct stoichiometry must be known. Another example is protein purification; during protein purification the quantity of protein present must be known to determine the yield1. The quantification of proteins is determined by methods known as protein assays. There are different protein assays used…

tertiary structure in detail ;consider protein shape, bonds involved, how these bonds are made (groups involved, location) give an example of protein having this structure. The primary and secondary structures are both present in a tertiary structure, the whole chain may then be folded further to give the protein molecule a complex globular shape that is known ate the tertiary structure. Similarly to the secondary structure the tertiary structure of protein is determined by a sequence of amino…