Protein

structure of proteins, emphasising the forces that sustain their three dimensional structures. Introduction MORE Proteins are nitrogenous organic macromolecules constructed from a base sequence of 20 amino acids at the ribosome in cells. There are over 100,000 different proteins encoded by thousands of different genes within the human body. They all perform different functions e.g. antibodies providing immunity and enzymes catalysing metabolic reactions. The determination of which protein is…

smaller. Different cells of multicellular organisms express different selection of genes depending on their specialized roles. An opening reading frame is any sequence of bases in whether DNA or RNA that could encode protein but doesn’t…

Report on Result and Discussion on isolation of protein from spinach leaves In this experiment, we tried isolating an actual protein called Rubisco from spinach leaves. The full meaning of these Rubisco means Ribulose-1, 5-bisphosphate carboxylase/oxygenase which is an enzyme that catalyzes the addition of a carbondioxide group to ribulose-1, 5-bisphosphate during sugar production in green plants. Although Rubisco is an abundant protein in plants, it is not the major component in all spinach…

Bio Lab- Denaturing Proteins Period B- Ms. Dann October 8, 2014 Nick D’Ambrosio, Matt Hermann, Carly Pruitt Title: Electrophoresis and Protein Weight Objective: The objective of this experiment was to gain a further understanding of gel electrophoresis, and to determine the denatured weights of unknown proteins using SDS gel. Hypothesis: If gel electrophoresis is conducted on unknown proteins, then the molecular weight can be determined by the distance travelled through the SDS gel compared to…

in kilobases) (1.2+0.7+0.4+3.1) = 5.4 kb. d) RNA Protein Truncation mutation in exon 2 Same length, same amount of RNA produced. Shorter in length (due to earlier stop codon), same amount of protein produced, usually changes the protein to non-functional, though not always. 3bp in frame deletion in exon 1 Shorter in length by 3pb, same amount of RNA produced. Shorter in length, same amount of protein produced, usually causes the protein to become non-functional, though not always. Splice…

Proteins are large, complex molecules found throughout the body. They are polymers made out of different types of small unites called amino acids. Each amino acid consists of a central carbon atom, which is attached to a hydrogen atom, an amino group, a carboxyl group, and a unique side chain or R group. There are twenty different types of amino acids and each one of them has different chemical properties. Amino acids are held together by covalent peptide bonds therefore the proteins, chains of…

1) A.) The specific DNA changes caused by the delta F508 mutation, include a production of the abnormal cystic fibrosis protein. This mutation can only be inherited if both genes in pair of DNA carry the cystic fibrosis mutation. The specific mutation functions as a chloride channel, that has an abnormal amount of salt and water balance. Normally a chloride channel would help to maintain the right balance salt and water inside a cell, but those mutated with F508 mutation, are imbalanced causing…

There are a number of drugs that bind to plasma proteins. The weak acids and neutral drugs tend to bind to albumin (the range of drugs binding is the widest) which is one of the most common proteins found in blood plasma. This binding to the plasma proteins can play a significant role in determining drug distribution. This binding also plays a role in drug dynamics in that only the unbound or free drugs interact with the receptors and penetrate the cell membranes. Albumin along with others (i.e.…

Elucidating the role of Heat shock proteins in poly glutamine (Poly Q) model in Dictyostelium discoideum . Introduction : Expansion of CAG trinucleotide repeats encoding poly glutamine (poly Q) tract in other wise unrelated proteins are responsible to atleast nine diseases . These diseases include Huntington’s disease (HD), spinobulbar muscular atrophy, dentatorubral pallidoluysian atrophy, and spinocerebellar ataxia (SCA) types 1, 2, 3, 6, 7, and 17.(1) Clinical features include ataxia and…

(there are other types but the ones mainly discussed in protein synthesis only involved these two). The DNA and RNA are partners that work together to make protein in protein synthesis. DNA is like the instruction manual to making the protein. The first step involves making the mRNA through a process called transcription. In transcription, a small portion of DNA is "copied" through coding to make the mRNA. Now entering the next stage of protein synthesis, the mRNA leaves the nucleus to go to…