Protein

Gel electrophoresis is used to separate DNA, RNA, and protein molecules by using an electric field. Protein gel electrophoresis is similar to agarose gel electrophoresis, but runs protein bands instead. The technique of protein gel electrophoresis uses cross-linked polymers of acrylamide (which is a neurotoxin and requires careful handling) or polyacrylamide to separate proteins and small nucleic acids. This technique takes longer than the agarose gels used to separate larger nucleic acids. Also…

attach to their complimentary nitrogenous bases forming a template, which is the process of DNA replication. Their findings were published in the scientific nature journal April 1953. Crick had made an important discovery to the understanding of protein synthesis by showing evidence that 3 grouped bases determined the type of Amino Acids formed. b. Rosalind Franklin was a famous chemist she gave crick and Watson the solution to their model by producing X-ray diffraction pictures showing that…

The proteins from each solution will then be precipitated by ethanol and suspended in 2-D cell lysis buffer (). Next, the proteins from the healthy volunteers and the sick volunteers will be stained with CyDye Cy3 and Cy5, respectively, and combined into one solution. The resulting solution will then be subjected to 2-dimensional gel electrophoresis. This will be done by transferring the mixed protein solution to isoelectric tube gel (pH 3-10) and subjecting…

Charbill Klayme 0993551 Dr. Hye-Jeong Yeo Functions of SREBPs Abstract SREBPs are 1,150 Amino Acid long proteins that are used to activate cholesterolgenesis. SREBP-1a are used to induce all the response genes. The SREBP-2 are used to allow expression of the LDL receptor. Ubiquination, usually by the kinase GSK3, can be the weapon to destructing SREBPs. SREBP-1a will build more cholesterol and fatty acids in rats and mice when it is over expressed. The SREBP-1c are best studied in the…

or tumor protein, is a gene that codes for one protein which stimulates another protein that inhibits enzymes needed to drive the cell cycle and functions as a tumor suppression. It is very important for cells in the role to suppress cancer. P53 has been described as "the guardian of the genome", referring to its role in conserving stability by preventing genome mutation (Strachan and Read, 1999). The name is due to its molecular mass: it is in the 53 kilodalton fraction of cell proteins. 2.…

wheat kernels in 150 μm sieve; Wwheat in 75μm sieve = weight of wheat kernels in 75 189 μm sieve; Wpan = 190 weight of empty pan; and Wmaize meal = weight of maize flour. 191 Determination of Crude Protein 192 The crude protein content of the roasted maize was determined in duplicate by Dumas combustion 193 method as described in AACCI Approved Method 46–30.01 (AACC, 1999) using a Leco, model 194 FP-528 (Leco Africa, Kempton Park, South Africa). Calibration of the instrument was done using 195…

2. The element being described is Sodium its symbol is Na it has an atomic mass of 22.989769. It has an atomic number of 11. This element has 11 protons, 11 protons, and about 11 neutrons. Protons have a positive charge, electrons have a negative charge, and neutron have a neutral charge. Electron shells are the outer parts of an atom containing the electrons. Valence electrons are electrons in the outermost shell. Valence electrons tell us whether an atom shares, transfers or receives electrons…

as, hormones are made of proteins, which are involved in respiration, digestion, reproduction, and normal vision. The structure of a protein is made of three-dimensional protein molecules. The first level is made of a linear arrangement of amino acids that creates the peptide chain. The second level is the hydrogen bonds that creates the pleated sheet or alpha helix, which is a coiled or spiral molecule (Mandal 65). The last level describes the overall structure of proteins, which are thin and…

Abstract : Proteins are vital components of all living cells and play a critical role in almost all biological processes. Protein structure identification is a significant challenging problem in computational biology. The Protein 's three-dimensional molecular structure (i.e., tertiary structure) reflects its proper function. Therefore, the identification of protein structure is a significant step towards understanding the protein 's function which is an important task to treat diseases,…

1.3 Identifying Knotted Proteins The complexity of protein folding makes it very challenging to fully understand the folding mechanism of proteins. More complex yet interesting challenges have been proposed. One of the most interesting challenges is the protein with knotted topology.(9-12) Knot is very common in our daily life. Sometimes we hate the knot and need to untie it. For example, our earphone cable often gets knotted and it takes forever to untie it. But, more often, we need the knot,…