Protein

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    as common. Finally, amino acid sequences of proteins…

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    TDP-43 Protein Analysis

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    According to T.F. Gendron et al., partial amounts of the toxicity are due to a gain in function by the protein. When this occurs, the TDP-43 protein has usually shifted its location from the nucleus to throughout the cytoplasm of the cell. This abnormal accumulation of TDP-43 in the cytoplasm is not only found in neurons and glial cells of the primary motor cortex but as well as in brainstem motor nuclei, the spinal cord, and in certain associated white matter tracts (Mackenzie et al 2010). It…

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    Hobert (2000) believes, that the patterns of these LIM-homeodomain proteins is evident from studies about Drosophila to C. elegans. LIM proteins have their own transcription factors and cytoskeletal elements. Structurally, a LIM domain has cysteines with 2 zinc fingers. These LIM homeodomain (LIM-HD) proteins regulate the formation of cell line cells and influence differentiation. (Dawid et al., 1995) To control these proteins, it is suspected that they all have a DNA-binding specificity due to…

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    three-dimensional structure of a protein not only defines its shape and size, but also its function. Proteins consist of a long chain of 20 amino acids folded up into complex shapes. Each nonpolar amino acid folds into the interior of the proteins during the folding stage, as they are hydrophobic. The primary and secondary structure determines the hydrophobicity of a protein. Each nonpolar amino acid folds into the interior of the proteins during the folding stage. For instance, membrane…

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    Protein synthesis is made up of two steps which are transcription and translation. The transcription part is when the information that is encoded in the DNA is taken away and it is the encoded in the mRNA and this information can then go to the cells that are within the nucleus and then onto in the cytoplasm. Then there is translation which is when the mRNA works with a ribosome and tRNA to be able to synthesize the proteins. Transcription The sequence of transcription starts with the Enzyme…

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    5. Protein purification There are many thousand kinds of proteins with different properties and functions in a cell. Proteins have been purified in active form on the basis of such characteristics as solubility, size, charge and specific binding affinity. Normally, protein mixtures are subjected to a series of separations, each based on a different property to yield a pure protein. 5.1 Separation based on solubility (Scopes, 1994) The protein solubility in various solvents depended on the…

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    Today I will be writing about the protein that I was assigned which was Structural Protein. What is your protein? What are they used for? They are used to build structural components of the body such as bones and cartilage. They are mostly found in larger cells. These proteins provide keratins, actin and support. I will be talking about why this protein is important and what does it do. The shape of a protei determines its function and there also a lot of different levels. An example of the…

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    Aleem Mohammad Stone Bio 2107 Lab 14 Sept. 2015 Accuracy Check of Protein Concentration vs FDA Labels Abstract The issue of food label accuracy is becoming very popular in the United States. The widespread awareness of genetically modified organisms and greater insight about the mass production of food lead to many questions and uncertainty about the labeling of edible products. The US Food and Drug Administration (FDA)’s accuracy in labeling will be tested in this experiment to see whether or…

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    DJ-1 Protein Analysis

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    The PARK7 gene is responsible for encoding the DJ-1 protein. DJ-1 is an oncogene, meaning it is a gene that can potentially cause cancer. That said, it is expressed in almost all cells and provides multiple intracellular functions. It can be noted that, DJ-1 protein provides extracellular protections, suggesting a role for DJ-1 as an anti-oxidative stress and molecular chaperone. In particular, the DJ-1 protein can function as a means to protect cells from oxidative stress.…

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    Alongside CRISPR-Cas9 in site specific genome editing are ZFNs and TALENs. ZFNs are a DNA-binding motif assembled as ββα that utilizes a roughly thirty amino acid protein with DNA recognizing amino acids at the alpha helix (Gaj, Gersbach, & Barbas, 2013). These groups of amino acids usually recognize DNA in segments of three. This does not present much specificity in a genome. Therefore multiple DNA-binding motifs need to be combined to create specificity and result in highly specific…

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