Protein

Literature Review: Green Fluorescent Protein and Histidine Tagged Proteins Histidine and green fluorescent protein (GFP) tagging are important in molecular biology because they allow for purification, tracking, and quantification of target proteins (Ferrari et al., 2004; Cho et al., 2011; Deponte, 2012). Histidine tagging provides a method for isolating and increasing the amount of target protein recovered from a biological organism or mixed sample of proteins (Masek et al., 2011; Singh and…

Separation of plasma proteins by molecular exclusion chromatography Sebastian Prisacariu 140013826 Introduction The components of blood is broken down into 3 parts. This breakdown is consisted of: Plasma which is a yellowish fluid that helps transport blood cells around the body and contains a small supply off proteins, platelets which are blood clotting agent, red blood cells which is a carry for oxygen and remover off carbon dioxide and white cells which is the bodies defence from infection…

Sequence 2 had the protein, presenilin-2-like, Equus przewalskii . The protein is also known as PSEN2 gene. This gene helps process proteins that transmit chemical signals from the cell membrane to the nucleus. When the chemical signals fire to the nucleus, they turn on genes that make for the cell to grow and mature. The specific role, presenilin is known for, is processing the amyloid precursor protein. The amyloid precursor protein is found in the brain and some tissues. Presenilin gets…

lie with their hydrophilic heads at the membrane surface and their hydrophobic tails on the inside..." (Anatomy and Physiology Textbook 66) There are two general membrane proteins, including the integral proteins, also known as transmembrane proteins. These proteins act as transporters, channels and receptors. "Integral proteins are…

Experiment 9 “proteins” and the purpose of the lab is to become familiar with some reactions of proteins and some of the tests used to detect proteins. The activities for this experiment includes the following: selected reactions of proteins will be carried out, several tests for proteins will be performed including: biuret test, xanthoproteic test, and millon test. This lab is broken down into two sections: precipitation (coagulation) of egg albumin and color tests for proteins. For the first…

The folding mechanism of knotted/slipknotted protein has become a challenging question over the last two decades.(10, 89, 90, 95, 99, 100, 173-175) Understanding how those proteins fold would be a great proceeding of the field of protein folding. Recent advances on protein folding shed light on this question using both experimental and computational methods. .(10, 89, 90, 95, 99, 100, 173-175) Most experimental studies on the folding of knotted protein have been limited to bulk experiments.(84,…

Protein X has a pI of 5.9, Protein Y has a pI of 7.6, and Protein Z has a pI of 8.5. For each of the methods below, describe how you would go about purfying Protein Y from Proteins X and Z (provide a pH that you would use and why.) Anion exchange chromatography: In anion exchange chromatography negatively charged ions will bind to the column. This will leave positively charged ions flushed through the column. By picking a pH of 6.5, protein X will flush through the column while protein Y and Z…

Organic Whey Protein What is Whey Protein? Milk can be broken down into two main proteins, casein and whey. Whey can be separated from milk and is considered a byproduct of cheese production. Whey is a complete protein because it has 9 amino acids that are essential to us because we cannot naturally produce these amino acids. Additionally, whey comes with a low lactose content which allows people with milk lactose intolerances to still be able to enjoy whey protein. What Can Whey Protein Do for…

Side effects of Whey Protein Sufficiently devouring protein is one of the necessities to assemble muscle, yet consuming a lot of it can build up a percentage of the known whey protein side effects. A typical individual has a necessity of something like 0.5 grams of protein per pound of his or her body weight. A man that is attempting to construct muscle requires all the more, around 1g every pound. Proficient jocks can take even 2 g every pound. Taking whey protein supplements can help you…

The question my group tried to answer was “Which protein solution contained protein, starch, and sugar, and if so how much?” The protein supplements we had were supplments 4 (Kroger Whey & Soy Protein- Vanilla),6 (Vega Protein and Greens) ,and 8 (Pure Protein Natural Whey Protein). We also had 3 reagents (Biuret’s, Lugol’s ,and Benedict’s). We had two different experiments, the first experiment was to get qualitative data. The second was to get quantitative date. The first experiment we tested…