Protein structure

The use of the steel frame structure in residential buildings has grown in the recent years due to the appreciation of the performance benefits, such as speed of construction, lightness of the material, safety, fire resistance, environmental benefits, such as reusability. The residential steel frame buildings, e.g. student accommodations, have benefits prior to other materials in terms of construction. Steel can be highly prefabricated, has spans of 5-7m (urban residential projects), with…

Objective: In-silico assessment analysis of Protein database entries of the Pf. lactate dehydrogenase (PfLDH) enzyme, considered as an important target for designing of anti-malarial drugs. Methods: PDB structures of the enzyme Pf. lactate dehydrogenase were downloaded from Protein Data Bank. These were subjected to ANOLEA energy assessment analysis and Swiss model was used to analyse Ramachandran plot. Results: The energy assessment analysis and analysis of Ramachandran plot were carried out…

microscope. 0.5nm Explain the appropriateness of light and electron microscopes for different purposes. (6) A light microscope can be used for objects as small as 200nm, they can resolve bacteria but cannot resolve viruses. They can reveal structures such as mitochondria, chloroplasts and the nucleus. Light microscopes are commonly…

material - DNA that makes up the genes and chromosome. The Ribosome: ~Made of dozens of different proteins as well as RNA, also found in large numbers in the cytoplasm of living cells. Ribosome are typically composed of two subunits: • Large subunit • Small subunit “ Free” Ribosome makes proteins that remain inside the cell, “Bound’’ Ribosome make proteins that are exported from the cell. ~Protein Synthesis ~Chemical reactions is molecules that are brought into contact with one another.…

for 30 min at room temperature. Then, trypsin/chymotrypsin- digested samples of KPNB1 obtained from the UV-crosslinking experiments with various concentrations of compound 1 were analyzed by nanoflow liquid chromatography and tandem mass spectrometry. The tandem mass spectra of the trypsin/chymotrypsin-digested KPNB1-compound 1 adducts showed 10 peptides coupled to compound 1 (Table S1, the Supplementary Information). Among these adducts, we found that the amounts of three of the products,…

Cellular Respiration takes place on the level of the cell, inside the mitochondria. This is the process of breaking down food in the presence of oxygen to form ATP. ATP stands for Adenosine Triphosphate which is an energy bearing molecule found in all living cells1. Things such as bacteria can perform this with their outer membranes. When a person runs, they use cellular respiration to create energy in the form of ATP which allows their muscles to move. Many other energy-consuming reactions of…

lie with their hydrophilic heads at the membrane surface and their hydrophobic tails on the inside..." (Anatomy and Physiology Textbook 66) There are two general membrane proteins, including the integral proteins, also known as transmembrane proteins. These proteins act as transporters, channels and receptors. "Integral proteins are…

are eukaryotic cells, or cells with a membrane-bound nucleus. Unlike prokaryotic cells, the DNA in a plant cell is housed within the nucleus. In addition to having a nucleus, plant cells also contain other membrane-bound organelles, or tiny cellular structures, that carry out specific functions necessary for normal cellular operation. Organelles have a wide range of responsibilities that include everything from producing hormones and enzymes to providing energy for a plant cell. Plant cells…

Abstract The purpose of this research is to understand how secondary structures of a protein, in this case sperm whale apomyoglobin, affect the denatured state of that protein. The scientific community has been working under the impression that once denatured by urea, a protein loses all of its secondary structure. In this research the effect that apomyoglobin’s history of secondary structure has on the denatured state of the protein will be determined through kinetics of the refolding…

Gel electrophoresis is used to separate DNA, RNA, and protein molecules by using an electric field. Protein gel electrophoresis is similar to agarose gel electrophoresis, but runs protein bands instead. The technique of protein gel electrophoresis uses cross-linked polymers of acrylamide (which is a neurotoxin and requires careful handling) or polyacrylamide to separate proteins and small nucleic acids. This technique takes longer than the agarose gels used to separate larger nucleic acids. Also…