Protein methods

mutation in one of ten genes (Varkie; et al., 2006). The most common mutations of epidermolysis bullosa simplex are associated with the K5 and K14 types of keratin. The K5 and K14 types of keratin are associated with very important intermediate filament proteins. When a mutation is present here, the basal keratinocytes become fragile and eventually rupture when acted upon mechanically. The K14 and K5 mutations are associated with epidermolysis simplex, which is the most common, but also the…

are very common diseases. Nuclear Factor (NF)-кB is a protein complex controlling the transcription of DNA. NF-кB is activated in tubules and glomeruli in various experimental models. Several studies have suggested that detection of NF-кB could be considered as a sign of renal injury. Objectives: This study aims to determine the association between NF-кB level and renal disease progression among patients with AKI and nephrotic syndrome. Methods: A comparative follow up study was conducted to…

RESULTS Phytic Acid The concentration of phytic acid was determined spectrophotometrically in three tested plant materials of Gishta as shown in table 2. The concentrations of phytic acid were obtained from 5 g dried leafy material. All the leaf samples contained phytic acid (detection limit 0.025 mg/ml) in this study. This may have been due to the fact that there was a limitation in terms of the detection concentration since concentrations less than mg was not tested. Seed extracts was found…

polyvinylpyrrolidone and inoculated 30 min in ice. This homogenate sample was centrifuged at 10000 rpm for 30 min at 4ºC and supernatant collected. The supernatant was assayed for Catalase (CAT), Superoxide dismutase (SOD), Malondialdehyde (MDA) and soluble protein with standardized protocol. For HM analysis, shoot and root samples were digested with 1:3 (v/v) HNO3: HCl and concentration determined by AAS. Efficiency of phytoremediation was determined by calculating bio concentration factor…

In this paper, the hypotheses being investigated were whether influenza A virus was capable of inducing apoptosis in mast cells, and if so determine if the virus was using this intentionally to its benefit and which pathways were utilized. The methods used included viral infection of cell cultures, hemagglutination assay, real-time quantitative PCR, plaque assay, transmission electron microscopy, TUNEL assay, flow cytometric analysis of apoptosis, western blot, subcellular fractionation…

molecules that act as a catalyst to significantly speed up the rate of the chemical reactions that take place within cells without being used up in the process. According to the website, www.ducksters.com, “enzymes are special types of proteins. Like all proteins, enzymes are made from strings of amino acids. The function of the enzyme is determined by the sequence of amino acids, types of amino acids, and the shape of the string.” Along with this we need to know about a peroxidase; “A…

separated by densities to form a pellet or supernatant after each centrifugation. This process is used to separate nuclei and mitochondria. Nuclei is a very dense organelle in the center of the cell coordinating the cell’s actions: growth, metabolism, protein synthesis, reproduction. The nuclei also will pellet after the 1st low speed of the centrifugation, which can be seen under a microscope. Mitochondria has a low dense organelle that has a double membrane, allowing oxidative phosphorylation.…

deoxyribonucleic acid, which is a self-replicating material that is present in just about all living organisms as the top chromosomes. DNA Isolation is the development of purification of DNA from a sampling applying a mixture of physical and chemical methods. Isolation is a routine process in molecular biology or in molecular analyses. Materials: • Electrophoresis chamber • Gel casting trays • Sample combs • Ethidium bromide • Loading buffer • Electrophoresis buffer • Transilluminator • DNA •…

The main goal of this study was to identify and analyze the D1S80 gene, and this experiment illustrated the versatility of these molecular biology techniques of PCR and gel electrophoresis to serve as methods identifying individuals. Both of the hypotheses made in this experiment that (1) there were genetic variation with the six students and (2) Student 3’s genotype was heterozygous were supported by the data. As seen in Figures 1 and 2 and Table 1 with different agarose gel percentages, it can…

Macro/micro nutrients, minerals, vitamins, essential fatty acids & water are the biomolecules whose optimum intake is necessary for health maintenance. 1. Food chemistry: Components like carbohydrates, proteins, fats, etc. are recommended with the help of food chemistry and also the deficiency caused due to possible physiological alteration. 2. Role of nutrients: The importance of vitamins, minerals, important fatty acids, & their contribution to health…