Protein methods

Electrophoresis Kendra Gibson November 17, 2015 A scientist drops DNA samples in small indentations at the end of a Jell-O-like gel. He turns on an electrical current at the other side of the gel. His anticipation grows as he watches the samples slowly move from one side to the other. He pulls out a ruler and makes some measurements. He steps back in surprise, for he had just identified the most feared serial killer in the world! He did this by using Gel Electrophoresis. Electrophoresis (the…

Enzymes are proteins serving as catalysts that carry out thousands of chemical reactions that occur in living cells. A catalyst speeds up a chemical reaction by changing the rate of the reaction without being consumed by the reaction. In an enzyme - catalyzed reaction the substance acted upon that is called substrate binds to the active site of the enzyme. An enzyme-substrate complex is formed held by hydrophobic interactions, hydrogen bonds and ionic bonds. The substrate is converted to…

Emily Astarita, Amelia Grose, Natalie Krapcho and I’s project is titled “Concentration Quantification and Comparison with the HIC Eluate and Bacterial Lysate.” The overall objectives of this experiments include using a spectrophotometer to obtain absorbance of standard solutions to create a standard curve from this data, and subsequently using the equation of the curve to find the concentration of GFP elute which was purified from the column. Using the spectrophotometer to create a standard…

also has a role on recycling the histamine neurotransmitter in Drosophila. In the synaptic terminal of a photoreceptor cell, histamine is released and enters the epithelial glia. From there, the ebony gene converts the histamine into carcinine. The protein carcinine is available for re-uptake by the photoreceptor cell where it can be reconverted to histamine by the tan gene. In a mutant tan gene fly, however, the photoreceptors have a decreased amount of histamines to release and low levels of…

Discussion The glycemic index of the dosa prepared from foxtail millet was low. This is due to the high levels of soluble dietary fiber in the millet. The total dietary fiber was reported to be27.02%, soluble dietary fiber 11.56% and the insoluble dietary fiber 15.44%11. It has been reported that the high viscosity of the soluble fiber delays digestion and absorption18. The glucose levels can be maintained to near normal with the help of dietary modification. It can be inferred from the above…

several subtypes of CYP450 family, CYP450 1A1 is going to be discussed. Consequently, the main of this paper is to describe the formation of enzyme structure, its active site, function, reactions with other compounds and diseases correlated to it. Protein Structure: Structure of CYP450 Three-dimensional…

4.1 Maintenance and sub-culturing of endophytic fungi The cultures were procured from already maintained repository in laboratory. The maintenance of the cultures involved preparation of Potato dextrose agar (PDA) plates, sub culturing of cultures and long term preservation. 4.1.1 Preparation of PDA (Potato dextrose agar) plates 39g of PDA was dissolved in 1000 ml double distilled water, stirred to mix properly and was transferred into 250 ml Erlenmeyer flasks and autoclaved at 121˚C at 15…

improved fluorometric leukocyte β-glucosidase assay for Gaucher’s disease. Clin. Chim. Acta, 115 (3), 369–375. 13. Wenger, D.A., Clark, C., Sattler, M., and Wharton, C. (1978) Synthetic substrate \s s-glucosidase activity in leukocytes: A reproducible method for the identification of patients and carriers of Gaucher’s disease. Clin. Genet., 13 (2), 145–153. 14. Beutler, E., Kuhl, W., Trinidad, F., Teplitz, R., and Nadler, H. (1971) Beta-glucosidase activity in fibroblasts from homozygotes and…

The inheritance of genes is vital to our existence. Genes are hereditary units containing DNA (deoxyribonucleic acid) and are passed from parent to offspring. The DNA provides instructions for the formation of proteins and as a result, genes are the main element responsible for the different traits we acquire as we mature. Genes are contained in chromosomes. There are 46 chromosomes in each cell (bar the gametes) and each chromosome contains a single long strand of DNA with thousands of genes…

activities of some viruses inside and also outside the epithelium cells. It also prevents the attachment of the antigens to the gut walls. The final sub-class of Ig is the E immunoglobulin (IgE). The IgE has been experiments by numerous functional methods. It is mostly induced by the virus. The IgE monomer has sedimentation. Its heavy chains consist of four constant domains (CH). Its structure is bigger than that of the IgG. The structure of the IgE has a fragment which is usually bonded by…