Protein methods

Introduction The study of this experiment was the Dopamine transporter gene. This gene is associated with different brain disorders like bipolar, as well as certain behavioural traits such as ADHD.[1] Dopamine transporter gene is a presynaptic plasma protein containing different VNTRs in it’s UTR and plays an important role in restricting the activity of dopamine by rapid reuptake into the presynaptic neuron. DAT is part of Na+ and Cl- dependent family with the addition of other…

It is also called anabolism or biogenesis. It is a enzyme-catalyzed method in which substrates are transformed into complex products. In this process simple compounds change or convert into other compounds or they join together to form macromolecules. Example: In this process gluneogenesis is responsible for the production of glucose from non carbohydrate originator. Actually biosynthesis is an enzyme-catalyzed process in which substrates are converted into complex compounds. 1. Biosynthesis…

The human body produces various essential proteins that provide functionality in the biochemistry of our system. How individual molecules are separated or a mixture of molecules are separated can be dependant of their size. Size exclusion chromatography (SEC), is the controlled separation technique that is based on the size of the particles in relation to the pore size (Barth and Saunders 2012). The separation process veers to be quick for large molecules and slower for smaller molecules. The…

Those with the disease can maintain appropriate levels of phenylalanine in the brain through monitoring with blood testing and subsequent diet adjustment. A diet low in protein and free from phenylalanine is important to maintain brain functions. (1) The brain requires amino acids including phenylalanine for building brain proteins and building neurotransmitters. (1,2) The more phenylalanine in the blood, the more that gets transported into the brain. This causes less of the other essential…

3.4.2.1 Test for milk:- 1. Determination of SNF in milk AIM: - to determine the SNF of milk Apparatus: - lactometer jar, lactometer and thermometer Principle: - It is calculated by using lactometer method. Procedure:-  Warm the milk sample to 40 to 45oCand maintain at this temperature till 5 min.  Mix the contents by rotating and inverting the bottle, taking care to avoid the formation of air bubbles.  Cool the sample to 15.5oC  Invert the sample bottle 2 to 3 times; pour enough milk into…

molecular similarity indices analysis (CoMSIA) studies on a series of benzo[b]thiophene-containing selective inhibitors of plasmodial NMT. Two QSAR models were constructed, one for Plasmodium falciparum NMT and another for Homo sapiens NMT, for each QSAR method. The predictive ability of all QSAR models was significant (r2 > 0.90 and q2 > 0.70). Comparing results of different enzymes, we achieved great understanding of structure-selectivity relationship. Finally, these QSAR models…

simply cytoplasmic membrane bound cells. The sphaeroplasts were then spun down using centrifugation, and the supernatant was removed, which presumably contained the contents of the periplasm. Once the periplasmic components have been collected, one method to obtain the cytoplasmic components is to lyse the cells, and then spin down any of the membrane fragments that are present (Thein et al., 2010); to do this we osmolytically lysed the cells by subjecting them to a hypotonic solution. We also…

Amylase Laboratory Report Introduction: Enzymes are a catalytic protein in a quaternary structure, that helps with the acceleration of molecules called substrates to achieve its desired chemical reaction. In this lab we used a specific enzyme called amylase. According to the lab manual, amylase is an enzyme found in one’s saliva or the pancreas to accelerate metabolism and cause a spontaneous reaction that helps with digestion of a substrate called starch. In order for any polymer such as…

much different from that of yellow soymilk. Purple soymilk has protein, ash, fat, carbohydrate and moisture content 2.76 ± 0.13, 0.12 ± 0.08, 1.17 ± 0.06, 1.27 ± 0.10, and 94.69 ± 0.04% respectively, while yellow soymilk from Tunde-Akintunde and Souley [35] has a protein content of 2.23-3.05%, ash content of 0.24-0.32%, fat content of 1.60-1.94%, carbohydrate content of 1.99-2.78% and moisture content of 92.02-93.29%. Black soybean proteins have the good amino acid score and high digestibility…

Introduction: All organisms depend on the action of enzymes to carry out the reactions of life. According to the text in Campbell Biology, enzymes are biological catalysts. These globular proteins speed the rate at which metabolic processes occur by lowering the activation energy or the energy barrier required to transform reactants to products. Each enzyme is specific, each containing active sites destined for a substrate. Substrates are the reactants or starting materials of chemical…