Protein methods

used to conduct the transformation process into Escherichia coli (E. coli). pGLO carries several genes such as β-lactamase, green fluorescent protein, origin of replication, and transcriptional regulator gene that affects transformation. For instance, the β-lactamase gene (bla) gives the transformed bacteria resistance to ampicillin. The green fluorescent protein gene (gfp) will provide the transformed bacteria with a green glow. The origin of…

Figure 3.1.2. Antibody titres of rabbit antisera. The antisera were collected from immunized rabbits and antibody titres measured by indirect ELISA as described under Methods (section 2.2.3.1). 3.1.2.1.3 Purification of α-LA, HRP and human erythrocyte specific polyclonal IgG The IgG fractions were purified from rabbit sera by ammonium sulphate precipitation followed by negative ion exchange chromatography employing DEAE-cellulose. The antisera were decomplemented, IgG fraction precipitated by…

 COMPOSITION OF MILK Milk is nearly complete natural food on the earth. It contains water, fat, protein, lactose and minerals as major constituents. The proportions of these constituents vary with species of the animal. For example, Mare’s milk contains only about 1.6% fat, while Reindeer’s milk contain 22.5% fat. Apart from this, the composition also varies with several other factors, viz., the breed of animal, the feeding pattern, season, lactation period, age of animal, environment, etc..…

the deleterious effects the gene causes when mutated, resulting in HGPS, can potentially help discover methods into reducing the accumulation of progerin in affected individuals of HGPS and, consequently, find ways into slowing down the process of aging in…

our project was to simulate the digestion of carbohydrates, lipids, and proteins. Using a certain medication called pancreatin we tested its effects on how it was able to break down these macromolecules. pancreatin is a mixture of pancreatic enzymes that help to aid in digestion; the enzymes that are present in this mixture include amylase (breaks down carbohydrates), lipase(breaks down lipids), and protease (breaks down proteins). We had set up trials for these enzymes to break down their…

Lab 1 – Ion Exchange Chromatography Danielle Small John Wright 20/01/2016 Introduction: This lab will use ion exchange chromatography – a process in which differently charged ions are separated, by altering the pH of the solution; which shifts the equilibrium of the molecules. During this lab we will separate two compounds from one solute that will be formed by amalgamating a ‘slurry’ – provided by the instructor. Resin or cellulose is used to create a sound support unit in order maintain a…

interpretation is transported out through atomic pores. Numerous proteins are integrated at the Endoplasmic reticulum. The ribosomes tie to the ER (a film bound organelle) and make an interpretation of the RNA message into protein that either navigates the layer (like a protein channel does) or is inside the ER in a vesicle (for solvent proteins that are emitted by the cell by exocytosis... like development elements and hormone proteins) (Meiner,…

amount of ions and water that enter and exit it. While some substances, such as, water, can enter and exit the cell through osmosis, others have to be transported more in some systematic ways, such as, packing or transporting different ions through protein molecule. (Flinders (2016)) Knowing the permeability of a cell membrane helps scientists on many circumstances. One of them is how much concentration of ethanol can damage the cells and how it can be prevented. From the journal Applied and…

which has four binding sites for biotin or biotinylated derivatives with extraordinary affinity. Biotin can be chemically coupled to a binder molecule (e.g., a protein, DNA, hormone, etc.) without disturbing the interaction with its target molecule by a procedure called biotinylation. Avidin can then be exploited to “sandwich” between the biotinylated binder and a targeting molecule or probe. Avidin was also coupled with a functional moiety in some cases. Through the avidin-biotin system, we can…

Denaturing Protein Gels Kaytlin Goodwin Cedarville University Introduction Electrophoresis experiments are conducted on proteins for the purpose of separating proteins based on their characteristics. The characteristics that can be examined during the experiment are dependent on the preparation of both the proteins and the gel medium through which they will run. Native gel electrophoresis experiments highlight and examine multiple protein characteristics, such as size, charge, and…