Protein folding

Q1) Each subunit in the protein consists of two domains – a large domain for the binding of ATP and a smaller domain for the binding of fructose-6-phosphate. Q2) The αβα sandwich are tightly packed together and are separated by a large interface from another subunit. Each domain contains several beta sheets in between anywhere between two to four alpha helices. Q3) Most of the β sheets are parallel, although there are a few antiparallel beta stands in the large domains. The α helices are…

What is Gene therapy? Gene therapy is the process of supplementing or replacing a gene in order to treat a medical condition. This type of therapy allows the body to make the protein that is not being made by the missing or malfunctioning gene. Introduction of new genes through vectors overrides the malfunctioning gene and contributes to normal functioning of the body. The process begins when the modified DNA is inserted into a virus vector. Then, the vector binds to the cell membrane. After the…

Introduction: One of the most common mass spectrometry–based proteomic methodologies used to day is the bottom up protein identification. First, the protein has to be digested into peptides using a protease. Then, the peptide will be submitted to the URMC Proteomics Resource and Laboratory for analysis using LC-MS/MS tandem mass spectrometry. In this approach, the peptides will be separated and introduced into a mass spectrometer using reversed phase liquid chromatography (RC-LC). This…

Dehydration Synthesis process and the Macromolecules. I’ll start with the Macromolecules. These molecules are the four molecules all living things need to survive. The four molecules are Proteins, DNA, Carbohydrates, and Lipids. Each of these molecules is made of Carbon, Hydrogen, and Oxygen at the very least. Proteins have Nitrogen as an added element, and DNA has Phosphate and Nitrogen. The base structure of each molecule has a carbon skeleton. This means that structures have Carbon as the…

of proteins in this fraction. For fraction 4, a higher concentration of imidazole was used to wash the solution, the gel showed the band migrated and stopped at 38 kDa. For fraction 5, highest concentration of imidazole was used and the migration stopped at 38 kDa as well. It represented the presence of purified protein in the fraction by showing only one band at the site, which indicated the protein was purified. The expected SpHTS protein was around 40 kDa. In the gel, the purified protein was…

structure and function of cellular components can be used as evidence for evolution. The two fields that contribute to molecular evolution research are molecular biology and biochemistry. Molecular biology is the study of cellular components such as proteins, carbohydrates, nucleic acids, and lipids. Biochemistry is the study of the chemical processes in organisms. These two fields of science act as evidence because they show that organisms…

our project was to simulate the digestion of carbohydrates, lipids, and proteins. Using a certain medication called pancreatin we tested its effects on how it was able to break down these macromolecules. pancreatin is a mixture of pancreatic enzymes that help to aid in digestion; the enzymes that are present in this mixture include amylase (breaks down carbohydrates), lipase(breaks down lipids), and protease (breaks down proteins). We had set up trials for these enzymes to break down their…

The aim of this paper was model the amino acid intake of broiler chickens for optimum economic in different scenarios with MC how to example. The second objective was described in software R the scripts for modeling the optimum amino acid intake for broiler. In addition, we considered the price of whole birds and DL-methionine cost according to Economic scenario. The dilution technique was used to allow formulates the experimental diets with the same balance of amino acids for all treatments…

receptor-mediated endocytosis, unlike the other two processes is a selective process; this consists of cell proteins in pits on the surface of the cell acting as receptors which bind with molecules and the cell will only absorb certain cells if they are bound to a particular receptor.…

Gene Therapy & Cancer Cells Gene therapy is often defined as a treatment that uses genes to cure or prevent diseases. Genes, DNA, and protein are all involved with each other, therefore, in many immune diseases, if one fails your whole immune system could eventually fail. This treatment is relatively new; hence it is still a pretty risky experimentation since it involves getting genes into cancer cells. Gene Therapy is often involved in serious cancer cell treatments; its main purpose is to try…