paragallinarum reference strains (HP1, HP 90 and HP144) and two Australian Av. paragallinarum reference strains (HP14 and HP60) were selected for growth curve construction. Three broths were tested for their suitability of growth, Haemophilus Test Medium Broth (TMB), Cation-adjusted Mueller-Hinton Broth (CAMHB) with supplements and Veterinary Fastidious Media (VFM). TMB was prepared with 1% Biosate Peptone (BD), 1% sodium chloride (Merck Millipore, Australia), 0.1% starch (Merck Millipore), 0.1%…
Oral Microbiome 10: Reflection 3 One a daily basis the human mouth can come in contact with many different materials and organisms. “The human mouth is home to billions of individual microorganisms, including viruses, protozoa, fungi, archaea, and bacteria” (University of Minnesota Department of Biology Teaching and Learning, 2016, p. 21). In specific, during this lab we are looking at Streptococcus mutans (S. mutans) and Lactobacilli. In studies, it has been shown that yogurt has helped…
Availability PosiGen has a fairly wide service area which includes New York, Louisiana and Connecticut. More specifically, Albany, NY, Baton Rouge, LA, Bridgeport, CT, Houma, LA, Metairie, LA and New Orleans, LA. If you live outside of these service areas and are interested in PosiGen 's services, you will need to contact them to see if they would be willing to serve your area. If you live in a state outside of PosiGen 's service area, then you would be better off finding a local solar company…
INT: Hello, I’m here with Lucy Brown who has just viewed, The Shawshank Redemption for the first time, and is willing to answer a few questions. Firstly, what qualities of Red and Andy’s characters do you believe brought them together? Brooke: Fantastic question. I believe that Red and Andy’s friendship developed majorly throughout their time in prison. Both Red and Andy started out as complete social opposites, Red from a poor background and Andy from a privileged white background. They both…
Three agar slants and three nutrient broths were inoculated with Staphylococcus epidermidis using aseptic techniques for transferring samples from broth, agar, and plate cultures. A sterile inoculating loop was used to transfer a loopful of culture from the culture to the sterile media. The lip of the culture tube and inoculated media tube was flamed before and after transfer. The inoculating loop was reserialized after transfer was complete. An agar deep transfer of S. epidermidis was also done…
Objective I. To learn how to inoculate the nutrient broth properly in preventing contamination to the nutrient broth and the culture Escherichia Coli. This is done by sterilized the loop and sterilized the neck of the tub with heat from before use and before put the cape on. II. To learn how to do a simple streak of an E. coli culture on a nutrient agar plate with one stroke from start to end like a “Z”. III. To learn how to do a complex streak where we grow E. coli culture on the nutrient…
(the tool): A wire made into a loop to assist in picking up inoculum from a culture of microorganisms. The loop is also used in the process of cultivating microbes by transferring inoculum for streaking. 3. Agar vs Blood Agar: a. Agar: a growth medium made with agar and other nutrients. The agar allows microorganisms such as fungi and bacteria to be cultured. b. Blood Agar: Blood agar is used for the purpose of cultivation and isolation of various strains of pathogenic bacteria. 4.…
Looking up at the night sky, little glowing dots are scattered across the night sky. They are known as stars. There are several billion stars in our galaxy, but only a few are seen with the naked eye. Stars are by definition, large exploding balls of gas made up of mostly hydrogen and helium. The two most unstable elements on the periodic table. The largest star in our galaxy is the sun. It’s the largest star and one of the few stars that are going through a star wide nuclear reaction. The…
Introduction : Enterotube II is used to identify multiple tests in one tube. twelve different conventional media plus a self-contained inoculating wire. The twelve test are for glucose and gas, then lysine, ornithine, H2S and indole, adonitol, lactose, arabinose, sorbitol, dulcitol, phenylalanine (amino acid), urea, and citrate (No VP test require). These medias performs 15 different biochemical tests that will determine the genus and the species of gram negative bacteria. This test will help…
The purpose of this experiment was to swab a chosen object, isolate a microorganism, and identify the microorganism through a series of morphological, physical, and biochemical tests. One chose to swab David Phelps’ pastor dreamer, which is one of the larger statues on The University of Oklahoma’s campus. The nose of this statue was swabbed and is where one’s environmental isolate originated. Through morphological, physiological, and biochemical experiments, one was able to gather results that…