Polymerase chain reaction

Discussion Alu is a transposable element that can be inserted into the human genome, enabling its use as genetic marker for ancestry differentiation,and thus identification. Due to new insertions, some humans do not have an alu insertions, while others may have accumulated mutations causing three types of polymorphisms, S1, S2 and L, the reference alu insertion, YB8 was useful as a standard to the comparison of nucleotide sequences changes to uncover the difference in the presence and absence of…

Agarose gel electrophoresis is a lab technique that is used to separate and identify DNA and RNA according to the size of the individual molecule fragments. It has many common uses in modern molecular biology such as separation of restriction enzymes, analysis of PCR results, the number of DNA in a sample, DNA sequencing, DNA finger printing, and forensic science. The basis behind agarose gel electrophoresis is manipulating the highly negative charge held by DNA. When placed in an electric…

Population Equilibrium of the AUG Sequence in the PV92 Region. Abstract – Alu sequences are found throughout the karyotype of humans and new world primates. Newer sequences that are roughly around one million years old can used to analyze human history since they are not yet stable in the population. One of these sequences that are still evolving is the Alu sequence on the PV92 region of chromosome 16. In this experiment we analyzed 29 individuals at Cameron University to find out how frequent…

Detection of Zika virus Introduction: Zika Virus, also referred to as ZIKV, has become a global health epidemic. The World Health Organization declared a Public Health Emergency of International Concern earlier this year due to the increase of areas affected by Zika. Originating in the Zika forest of Uganda, ZIKV was predominantly found in areas of Africa and Asia. However, there have recently been reports of more than 36 countries with cases of the virus. (Chen, 1), (CDC) ZIKV is a single…

Introduction: Bacteria are very diverse and are part of the prokaryotic domain. Some bacteria can be harmful, but others can also be beneficial in many ways. There are bacteria everywhere on Earth; places that you would think would have bacteria, like your gastrointestinal tract or our skin (Wenner, 2007). Although bacteria are small in size and are a simple cell organism, it is essential to life. There are different ways in how to distinguish and classify bacteria. One way is you can look…

We also put every set’s kiwi and extraction solution mixture into the water bath together. And in between performing the extraction for each set, the other sets’ was resting and the enzymatic reactions might have started to take place that then destroys the DNA and makes it less intact together after ethanol is added . There are enzymes called DNases found in the cytoplasm and its function is to destroy other DNA’s from virus or sicknesses that…

Group report Genotyping of Human ABO Blood Group Molecular Biotechnology Fong Fuk Ka (140280041) Liu Ka Hin (140123508) A. Results and analyses 1. Show your gel photos (PCR and RFLP) with clear figure numbers, titles and legends. 1 2 3 4 5 6 7 8 Figure 1 PCR products of two partial segments of ABO locus in human DNA shown on agarose gel from group 6. Well 1 was blank, well 2 was 100bp ladder, well 3 was own sample (5/6), well…

Emily Astarita, Amelia Grose, Natalie Krapcho and I’s project is titled “Concentration Quantification and Comparison with the HIC Eluate and Bacterial Lysate.” The overall objectives of this experiments include using a spectrophotometer to obtain absorbance of standard solutions to create a standard curve from this data, and subsequently using the equation of the curve to find the concentration of GFP elute which was purified from the column. Using the spectrophotometer to create a standard…

Analytical agarose gel electrophoresis This molecular tool is mainly used to separate and identify DNA fragments and in some cases also to purify certain DNA molecules that can be cut out of the gel after the electrophoresis. DNA molecules are negatively charged, and by loading them into an agarose gel and applying a current creating a uniform electrical field, these molecules will move across the gel. How far they travel, depends on their electrophoretic mobility. Size, structure and total…

DNA Gel Electrophoresis Electrophoresis is a process in which macromolecules are separated by utilizing their electrical charge and size. A technique for separating protein molecules of varying sizes in a mixture by moving them through a block of gel, as of agarose or polyacrylamide, by means of an electric field, with smaller molecules moving faster and therefore farther than larger ones. Gel electrophoresis of DNA is used in DNA profiling. When DNA is found at a crime scene, it's sent to a…