Polymerase chain reaction

special bodies that allow it to work in low level regions in which wouldn’t be supported elsewhere inside of the specific cell. Peroxisomes break down chains of long fatty acids, amino acids, etc. that are vital for the normal function of the brain and body. Peroxisomes are found in the cytoplasm as well. They convert acids to carbohydrates. Reactions toward peroxisomes produce a very harmful…

Report on Result and Discussion on isolation of protein from spinach leaves In this experiment, we tried isolating an actual protein called Rubisco from spinach leaves. The full meaning of these Rubisco means Ribulose-1, 5-bisphosphate carboxylase/oxygenase which is an enzyme that catalyzes the addition of a carbondioxide group to ribulose-1, 5-bisphosphate during sugar production in green plants. Although Rubisco is an abundant protein in plants, it is not the major component in all spinach…

How to Make a Lava Lamp Don’t you think lava lamps are really cool decorations? Believe it or not they’re wq2actually really easy to make. I’m not talking about the kind you plug in, but this DIY lava lamp still works and it's awesome! It can glow in the dark. You will definitely love this glow in the dark lava lamp! Materials needed A clear glass or a hard plastic bottle with a lid Vegetable oil or corn oil ½ cup of water Food coloring 2 large glowsticks Scissors or knife Alka- Seltzer…

This thesis mainly focused on analysis approaches of twenty potential isomeric and designer drugs of 1-pentylacylindoles series of synthetic cannabinoids compounds associated mainly with naphthoylindoles and benzoylindoles structures, via using various analytical methods. These isomers and analogs represent potential designer modification of banned drugs and show the same elemental composition and yielding identical nominal, exact masses and general analytical properties which make the potential…

Sodium bicarbonate (NaHCO3), or sodium hydrogen carbonate, also known as baking soda and bicarbonate of soda, is a soluble white crystalline compound, with a slight alkaline taste resembling that of sodium carbonate. It is found in many mineral springs, in natural deposits in USA and is mainly produced by the Solvay process: NaCl + NH3 + CO2 + H2O NaHCO3 + NH4Cl Sodium bicarbonate, when exposed to an acid, releases carbon dioxide and water: NaHCO3 + HCl NaCl + H2O + CO2 (gas) Above 70°C,…

To first perform this experiment a beaker of water was set to boil on a hot plate while samples of serum and benedicts reagent were collected. Six test tubes were gathered and labeled by which blood vessel the serum was collected from and by which category, either postprandial or fasting. Then by using a metric ruler two measurements were placed on the bottom of each tube, one at one centimeter and one at two centimeters. This was to indicate how much serum and Benedict’s reagent to use,…

change in the blended mixture by putting a small amount in well plate and putting the same amount of water in the container next to it. We put three drops of the solution and mixed it thoroughly with a mixing stick. While we were waiting for the reaction, we decided to test the change with a hot plate. We put…

DISCUSSION OF RESULTS: Answer the following Questions: 1. How did changing the angle of the slope affect the acceleration of the object? Use information from the summary table and/or graph. According to our data, the larger that the slope was, the faster the acceleration of the object was. Although, this was not the same for the 30° angle and the 40° angle. This may have occurred due to an error in recording the data or a bad ticker timer being used. 2. What forces are acting on the object…

A single Lactaid pill was grinded with a mortar and pestle. 120 mg of the ground up Lactaid pill was dissolved in 200 mL of 0.08 M phosphate buffer (7.7 pH) and the resulting solution was centrifuged to remove any capsule remains. Enzyme and Substrate Solution Preparation A blank tube was prepared by adding 5 mL 0.08 M phosphate buffer (7.7 pH) and 0.5 mL of 2.5 nM ONPG to a cuvette to zero the spectrophotometer with the spectrophotometer set to 420 nm. The substrate solutions were prepared…

Activity 2: Chemistry In a Bag With Chemistry In a Bag, students learn the difference between a physical change and a chemical change and how changes can either be endothermic or exothermic. Set-up: Each group of four students will need 1 canister filled with calcium chloride (CaCl2), 1 canister filled with sodium bicarbonate (NaHCO3), 1 dropper of phenol red, a set of measuring spoons, a measuring cup, a portion cup, four pairs of goggles and four sandwich bags. Make sure there are paper…