Where Do Bacteria Grow? I had some prior knowledge about bacteria before conducting the environmental sampling lab. I knew that bacteria could be found in many different places. Also, different types of bacteria grow in different conditions. From prior experience, I remembered that to grow bacteria in a petri dish one must follow the proper technique. I chose to test how much bacteria grows on toilet handle in the bathroom. My prediction for the experiment before it was conducted was that…
The oldest forms of life are single-celled organisms known as microbes. There are many different types of microbes that are found all around the world, and they play an important role in the environment, in the body of animals, and even in the health of plants. The specific type of microbe investigated in this paper is the gram positive bacterium known as Kocuria rhizophila, which has a bright yellow color that simplifies identification. The main objectives of the paper are the following:…
the experiment. This included, a microscope, three glass microslides, alcohol, caffeine, and water, four pippets, then finally three water fleas. After our workspace was ready we took the first flea and placed the flea on a glass plate then carefully latched the plate onto the microscope. Once the third team mate located the water flea on the microscope, I placed one single drop of water from…
liquefied. Then pour agar inside the petri dish, about half full. Make sure agar is fully liquefied, or it will have bubble spots in agar. Make sure all four Petri dishes are half full. Seat agar plates out for room temperature for about one to two hours, end then place them in the refrigerator. Agar plates should ready and use in about a week…
microorganisms. One of the microorganisms is gram positive which can be separate from the gram negative using the four-way streak isolation from the lab manual. The purpose of the streak isolation is to obtain single colonies on a Columbia CNA agar plate. C-CNA agar is a type of…
MacConkey agar plate, and then streaked once more on a separate MacConkey agar plate. Two plates were used in an effort to ensure that isolated colonies were produced. The aseptic technique was also used, and the loop was sterilized in the bactoincinerator after each streak for the same reason. The two MacConkey agar plates were placed in the incubator (35oC) and then retrieved 24 hours later. Upon retrieval, the two plates were gathered and isolated growth was seen on both plates. However,…
Table of Contents: Introduction………………………………………………………………………………………………………………………..2 Aim……………………………………………………………………………………………………………………………………….2 Background Info……………………………………………………………………………………………………………………2 Hypothesis…………………………………………………………………………………………………………………………….2 Variables……………………………………………………………………………………………………………………………….2/3 • Independent…………………………………………………………………………………………………………………………..2 • Dependent……………………………………………………………………………………………………………………………..3 •…
We first evaluated whether Honokiol treatment was toxic to the APP-CHO cell line. Cells were treated with various concentrations of Honokiol (0.5 μM, 1 μM, 2 μM, 5 μM, 10 μM and 20 μM) for 24 h and cell survival was determined by Prestoblue® assay (Fig.1A). As shown in Fig.1A, when exposed to Honokiol concentrations of 10 μM or lower, the viability of APP-CHO cells was the same as untreated control cells. However, a significant decrease in cell viability was observed with 20 μM Honokiol…
The End Zone: Measuring Antimicrobial Effectiveness with Zones of Inhibition Introduction: A Kirby-Bauer test is a microbiological method used as a form of testing bacterial resistance, it’s also known as a Zone of Inhibition test (1). The way in which the Zone of Inhibition was determined in this experiment was by measuring the diameter of the resisted zone in 3 areas and taking an average. As numerical data was required for this it means that in this experiment the Zone of Inhibition Test was…
Method: Purpose: To investigate how Porcellio Scaber reacts in terms of orthokinesis to different humidities in their habitat. Equipment- 75 slaters which range from 5-10mm in length (kept in a plastic container with holes in the lid, with dirt and bark so that they are kept in a natural environment as possible so can react naturally during experiment.) 5x petri dish set ups labelled from 50-90% (One petri dish on the bottom will contain glycerol and have a mesh/gauze hot glued over top…