Plate tectonics

2.5. ANTIOXIDANT STATUS 2.5.1. TLC - Semi Quantitative DPPH Assay 0.2 % DPPH solution in methanol was prepared and kept in the fridge for further use. The grid space was marked with 1.0 cm2 space on an aluminum based TLC sheet (Merck silica gel 60F254) and a stock solution of all the extracts together with the standard were prepared in methanol. A series of dilutions of the stock together with the standard were prepared ranging from 400 µL to 0.01µL for the last dilution. The grid on the TLC…

Wash hands and spray down or wipe your work are with disinfectant. Dry off with paper towels. Label the bottom of your agar plate (petri dish) as diagrammed in your Experimental Design (note: the bottom of the plate contains the nutrient agar, which looks like a clear/beige gelatinous material). Determine your two sources of collection for bacteria specimens. When you have decided, write them in tour data table on your…

Blood Culture Contamination For the purpose of this research, Archbold Medical Center Laboratory is the place I chose to conduct my research. Blood Culture contamination is a big problem not only at Archbold, but at many hospitals. The problem is not only within the laboratory, but from outside sources also. Although contamination is a problem, it can be easily fixed. Whenever a blood culture is ordered by a physician, we can assume the patient is experiencing some type of bacterial infection…

To begin, a streak was performed on two TSA plates and a blood agar plate using the three-sector streak technique from the unknown culture tube to isolate the two unknown bacterial colonies. The plates were then incubated for 12 hours at 37 ̊C. The blood agar plate was used to create a greater contrast in color to help distinguish between bacterial colonies by size and color. Two distinct colonies could be seen growing on the blood agar plate shown in figure 1. The smaller, opaque colored…

antigen binds to the coated antibodies. The excessive use of reagents to coat and wash the 96-well plate, and the 96-well plates it is self where the assay is performed on. Therefore, this article introduces 3D printing platforms for ELISA 96-well plates. With 3D plates, sensitivity of the assay increased as well as antibody binding time to the 96-well plates bay 2 to 3 folds of the traditional ELISA plate would take. This 3D 96-well pate provides a potential use of reduced volume of reagent and…

Question: How much bacteria will be found in each pedicure spa? Hypothesis: If I swab multiple pedicure spas using a test kit, streak plating, and colony morphology, then I will find the presence of bacteria is unsafe because cleaning practices are not followed properly. Procedures Determine the five pedicure spas in which you will obtain samples from Give each pedicure spa an anonymous name (Ex: Spa A, Spa B, Spa C) Type up an approval letter stating what you will be doing and what it is for…

of flow. After the pressure difference is generated, the fluid is passed through a pressure recovery exit section. In this section, the 80% of the differential pressure generated at the constricted area, will be recovered. However, in the Orifice plate, all of the pressure loss is not recovered because of friction and turbulence losses in the…

3.1 The isolation of Aeromonas hydrophila When using selective medium developed by Rimler and Shoots (1973), we obtained 95 bright yellow colour isolates with white edge which lead to the alleged as A. hydrophila. Of 95 isolates were further screened using SNI 7303 (2009) methods which produce 56 isolates. The isolates were further screened using Dorsch et al. (1994) and Cascón et al. (1996) method. 3.2 Bacterial DNA isolation and Identification. DNA amplification using Dorsch method…

The purpose of this experiment was to examine the different growths of minority organisms within a variety of media cultures. These cultures included types of selective, differential, and enriched media and were applied to split sections on the agar plate. Each of the four broth cultures: Escherichia coli, Enterobacter aerogenes, Staphylococcus epidermidis, and Streptococcus faecalis showed observable growth when applied. The different medias will either promote or inhibit the growth of the…

Once completed label the bottom of the blood agar plate (not the lid) with the details of sampling area e.g. Name, Date, Location, Sampling Area. Complete the procedure 2 more times in either a clinical or a non-clinical area. Part 2: Cross-Infection Control Part A Method: Sit at a bench with elbows on the bench, head resting upon the hands and poised directly over an open blood agar plate. Recite (with enthusiasm!) the following incantations 10 times, emphasising…