Plate tectonics

Three agar slants and three nutrient broths were inoculated with Staphylococcus epidermidis using aseptic techniques for transferring samples from broth, agar, and plate cultures. A sterile inoculating loop was used to transfer a loopful of culture from the culture to the sterile media. The lip of the culture tube and inoculated media tube was flamed before and after transfer. The inoculating loop was reserialized after transfer was complete. An agar deep transfer of S. epidermidis was also done…

loop and sterilized the neck of the tub with heat from before use and before put the cape on. II. To learn how to do a simple streak of an E. coli culture on a nutrient agar plate with one stroke from start to end like a “Z”. III. To learn how to do a complex streak where we grow E. coli culture on the nutrient agar plate for differential the isolate colonies presence. IV. To learn how to know whether the culture is a selective or differential media. The culture bacteria that will be using are…

do to the loop in between streak patterns (on the same plate)? a. After your first streak pattern you should flame the loop again and let it cool. Then you follow the same procedure of lifting the Petri plate, streaking it across the first streak pattern onto the second quadrant of the plate, flame the loop again, etc. 6. How much of the plate do we cover with the primary inoculum? a. We should cover between a quarter and third of the plate in total…

The purpose of this experiment was to swab a chosen object, isolate a microorganism, and identify the microorganism through a series of morphological, physical, and biochemical tests. One chose to swab David Phelps’ pastor dreamer, which is one of the larger statues on The University of Oklahoma’s campus. The nose of this statue was swabbed and is where one’s environmental isolate originated. Through morphological, physiological, and biochemical experiments, one was able to gather results that…

microorganism produced bubbles. This means that this microorganism produces the catalase enzyme. In order to determine if the microorganism can tolerate high salt environments, it was streaked on an MSA plate. The microorganism grew on the plate indicating that it can grow in high salt concentrations. The blood agar plate was used to test the microorganism for hemolytic…

present of melatonin 0, 20, 200 and 2000 pg/ml under both 5% O2 and air condition. Samples were diluted 100 fold in PBS and analysed in duplicate with standard (0, 0.5, 1, 2, 5, 10 and 20ng/ml), blank and quality control. To avoid variation among plates, all samples were run together. Inter and intra assay % CV was (11.1) and (5.1) respectively. 4.2.3.2 Oestradiol…

helped lead to the identification of the unknown. For this procedure, you grab a Blood Agar plate, and then streak the plate with a flamed loop containing your unknown. After the plate is streaked, you will then ask your instructor to place antibacterial discs throughout the plate. These antibacterial discs, after growth, will show the zone of inhibition which will then lead to your results. You leave the plates for a couple days in order to later on see some growth. My results were…

Introduction Bacteriophages, informally known as phages, are a specific type of virus that only infect bacteria. Since their discovery in the early 1900s , researchers have began studying the various behaviors and interactions of phages within the environment (Poxleitner, Pope, Jacobs-Sera, Sivanathan, & Hatfull, 2016). Their name, bacteriophages, is derived from the idea that they are “bacteria eaters.” Due to their bacteria-destroying nature, they have been used in phage therapy in which they…

ABSTRACT: This study was carried out to evaluate the effect of pretreatments prior different drying methods and temperatures on compositional quality of the dried mushroom. Oyster mushroom (Pleurotus ostreatus) was cultivated on rice substrate and collected samples were pretreated (steeped or blanched) by different solutions (0.1% sodium metabisulfite and 0.1 and 0.5% citric acid) and dried using oven at 50°C and 60°C, sun-drying at 30-35°C and microwave drying at 2.45 GHz until reached to…

incubation , the plates with fungal colony were flooded with 1% iodine and 2% potassium iodide. The appearance of clear zone surrounding the colony was considered positive for amylase enzyme. Protease activity Protease assay was performed by growing the endophytic fungi on GYP gar media amended with 1% skim milk and pH was adjusted to 6.5…