Pipette

study. To begin PCR analysis, start by taking six tubes (labeled 1-6) that contain PCR reaction mix. Three of the tubes have primers, for each of the tubes with primers touch a pipette tip to one of the colonies that grew on the tetracycline labeled plate and dip into the tube containing the primer. Change the pipette tip and do the same for the other tubes and plates. Bacteria from one plate is put into one tube. Do not mix the samples of bacteria. The other three tubes do not have a…

pick up the spill. Viva triumphed over what many people would believe the best paper towel brand, Bounty. One source of error in the experiment was the lack of precision in measuring water. To improve the experiment, I could have used a special pipette that would measure more precise volumes of water. A second source of error was in introducing paper towel to the water. While placing the paper towel down onto the water, the amount of force I used varied for each trial. To combat this, a…

Titrating Vinegar with Sodium Hydroxide (NaOH) Introduction Acids are anions that have at least one hydrogen cation tacked onto their molecular formulas. The Bronsted definition of an acid states that acid is a proton, H+, donor.3 Acids can fall into one of two categories: they can be strong or weak. Strong acids completely dissociate their H+ and weak acids only partially dissociate their H+.3 This means that a strong acid cannot return to its initial state once it undergoes a reaction. On the…

each test. Performing Gel Electrophoresis 1. Gather all materials needed. You will need electrophoresis chamber, power source, molds, Tris-Borate-EDTA 5x Buffer, DNA samples, and micro pipette. 2. Take the mold out of the mold plastic. 3. Place the gel mold in the electrophoresis chamber. 4. Using the Micro-Pipette fill the wells in the mold with the DNA sample. 5. Pour the buffer into the chamber ( do not pour directly onto the mold). Pour until it is about 1 to 1 ½ ml over the gel. 6. Plug…

intracellular recording technique consists of puncturing a prepared cell with a conductive pipette. The difference in potential between the extracellular electrode and the wire in the pipette is recorded and displayed on the voltage vs. time graph. Figure 1 provides an example of an intracellular voltage recording trace for a muscle fiber cell. The dip in the voltage graph occurs after the insertion of the pipette into the cell and reflects the resting membrane voltage of the…

flask, other materials included the still head, 10 mL graduated cylinder, 2 pipettes, support plate, the long distillation column, rubber connector with support rode, 2 ring clamps, an additional rubber connector, copper turnings, sand bath, ring stand, a thermometer adapter, and a thermometer1. Before assemble of the distillation apparatus could begin, the 50/50 mixture of ethanol and water needed to be prepared. Using a pipette, 2 mL of water was…

the nutrient agar plate was coated in culture. The plate was left to incubate at (Ryerson Department of Biology, 2017). Materials used in Experiment 1.6: Escherichia coli (E. coli) culture, 5 1mL microtubes, 10 mL sterile saline, sterile 1.0 mL pipettes, pi…

We added one drop of blood to the test tube and twirled it and tenderly flicked the base of the tube to blend it. Utilizing an alternate pipette we put one drop of NaCl/blood arrangement onto a magnifying lens slide and put a cover slip over it. We took a gander at this under the magnifying instrument and recorded our perceptions. We rehashed this same procedure with 0.45% NaCl arrangement…

distilled water. Reaction for Oxalic acid and Sodium hydroxide: C_2 H_4 O_4+2NaOH →〖Na〗_2 CO_4+2H_2 O (The ratio between Oxalic acid and Sodium Hydroxide is in the ratio 1:2) For carrying out titration take 25ml NaOH in a conical flask using a pipette and fill up the burette with 0.1molar Oxalic acid. Add 4-5 drops of colorless phenolphthalein in the flask containing NaOH. Note the titer value for 5 five trials and take the average titer value into consideration. Perform titration between…

Introduction The topic of the lab was to introduce the basics of enzymes. The topic is important because they are in every living cell and because of enzymes chemistry is allowed in the cell. They do this by providing the energy needed for chemical bonds to break down and lets molecules interact with each other. For part 1 of the lab my hypothesis was if you increase the concentration, then the absorbance will also increase. For part 2 section A my hypothesis was if you increase the…