Pipette

donor is an egg donor, i.e. a female. The egg needs to be in the stage at which fertilization would occur if it was to be fertilized in sexual reproduction. Firstly, the nucleus of the egg cell (which has not been fertilised) is removed using a sharp pipette. This process in known as enucleation. Next, the nucleus of the donated somatic cell is also removed and transferred into the egg cell. For the cell to then begin division (mitosis), chemicals or electric pulse is applied to stimulate…

Introduction Enzymes are specialized proteins that act as catalysts. Enzymes are a key part of many biological processes because they are capable of lowering the activation energy of a chemical reaction. By lowering the activation energy enzymes are able to speed up reactions allowing for multiple chemical processes to occur at a faster rate in many living organisms. Enzymes work by binding to specific molecules called substrates. The enzyme observed in this experiment was amylase and its…

Chemical kinetics are governed by the mathematics of systems of differential equations (Thermodynamics and Kinetics). This means that the rate of any chemical reaction is determined by the type, and amount, of reactants present. Note the rate of a reaction is how fast it occurs. Furthermore, such rate equations can either be distinctively linear or non-linear when graphed. Non-linear equations are supposedly more complex as they generally react to small changes within its parameters…

A chemical reaction is the change of a molecule from one arrangement to another. Our whole bodies are made up of cells, which are chemical systems. To sustain life there must be chemical reactions all throughout the body (Barnhart and Hopper 2011). At what rate the reaction can occur is dependent on the activation energy. Activation energy is the amount of energy the reactant molecules must surpass for the reaction to occur (Mathew Bui et al. 2006). Temperature and catalysts are the most basic…

Extraction of alkali from banana peels is known to depend on such parameters as ashing temperature, ashing time, soaking time and particle size of the ashed sample, soil type and variety. Alkali was extracted from three Nigerian grown banana peels namely: Saaro, Oloosun and Paranta for the purpose of determining the concentration of the extracted alkali. The concentration of the extracted alkali ranged from 0.02 to 0.10mol/dm3. The three varieties of the banana peels gave a maximum concentration…

Introduction Affinity Chromatography, also referred to as biospecific chromatography, is a technique of partitioning rooted in biological affinity (Dean). This practice capitalizes on complex interactions such as bond formation between enzymes and substrate, or more broadly: macromolecules and ligands. Purification is at the center of the use of affinity chromatography. In general, samples may be run through a gel that contains a corresponding component that binds with the sample at certain…

Introduction In 1950 the Nobel Prize for Chemistry was awarded to Otto Diels and Kurt Alder for their description of an invaluable chemical reaction (The Nobel Prize in Chemistry 1950). The reaction they described was for the formation of carbon-carbon bonds in a six membered ring with exceptional control of its stereochemistry in one step (Diels-Alder Reaction Gains Nobel Prize). This was an important discovery due to the fact that six membered rings are abundant natural products and man-made…

Unknown Bacteria 228 Adisney Pino Professor Dowding Miami Dade College Section T/R 7:05PM Introduction Enterobacter aerogenes is rod-shaped bacteria that belongs in the family of Enterobacteriaceae. This rod-shaped bacterium is gram negative which makes it a facultative anaerobe which grew perfectly in 37-degree temperature. This species was motile and therefore it can synthesize an enzyme known as ornithine decarboxylase. This bacterium is prevalent in the intestines of animals…

Carbohydrates are macronutrients which can be found in all types of food. A carbohydrate molecule contains carbon, oxygen, and hydrogen (Yacoub, n.d.). When found in food, carbohydrates can be starch, sugar, and fiber. Carbohydrates can either have simple or complex forms. For example, glucose, a sugar, is a monosaccharide; these are carbohydrates in their simplest form (Timberlake, 2006). When only a few of these monosaccharide are linked together, they create oligosachharides. If many of the…

With this information, we must adjust the [Pb] ppm, as adjusting the volume of the pipettes to match the [Pb] ppm is not a possibility. We know that 1 ppm is equivalent to 1 mg/L, thus we can re-evaluate our [Pb] ppm values from the same equation as above: With this information, we can complete the Table 3 for Lab 5 found in the Lab Manual:…