Pipette

This is required essential because the volumetric pipette is being used to transfer 20.0 mL of 1.00 molL-1 Sodium Hydroxide into an Erlenmeyer flask to be diluted. By rinsing it with water first it ensures that the sodium hydroxide wont react with anything in the volumetric pipette and be diluted. When creating the 200 mL of 0.100 molL-1 solution it is assumed the sodium hydroxide is 1.00 molL-1, with this information…

is to determine through calculations the concentration of acetic acid content within a common white vinegar. Apparatus / Materials Plastic Tray Electronic scales Sodium hydroxide Volumetric flask and lid Glass funnel Distilled water Pipette Volumetric pipette/s (5mL, 20mL) Bulb Conical flask Retort Stand Burette Burette clamp White Tile Method Place the plastic tray on the electronic scales and press tare to set the wither to zero; Weigh out 0.7993g of Sodium hydroxide in the plastic…

This lab began by calibrating a Pasteur pipette using hexanes. After adjusting and turning on the spot ventilation, approximately 5 mL of hexanes was measured and placed in a clean and dry test tube. A clean, dry test tube was also placed beside, in which to keep the pipette when it was not in use. The test tube of hexanes was kept stoppered when not in use. The pipette was used to measure exactly 1mL of hexanes into a graduated cylinder, with the pipette being held vertically and the number of…

temperature column. Once the 5 minutes are up, without removing the tubes from their temperature baths, use a pipette to transfer 3 drops of the starch solution from each temperature treatment to the 0 minutes row where the iodine was recently placed. Label each of the transfer pipettes with the corresponding temperature so they can be reused for each time interval but make sure to use a separate pipette for each temperature treatment. During the temperature treatment, pour the starch solution…

methods, do differ a lot from one another. One of the reasons for this huge difference, was the use of a serological pipette instead of volumetric pipettes. For all transfer of solutions where a pipette was needed, a serological pipette was used. Now this could be one of the main causes of error in the data, because a serological pipette is not accurate, however a volumetric pipette is. This mistake may have also caused a correlation coefficient that’s not close to 1 or -1. And the concentration…

Whitehouse The first part of the experiment consisted of finding the accuracy and precision of three different measuring devices, a 50mL beaker, a 25mL graduated cylinder and a 25mL pipette. The mean volumes and uncertainties were found to be 22.5mL 1.9, 23.72 .25, and 24.89 .43, respectfully. The 25mL pipette was found to be the most accurate out of the three. The density of water was found to be 0.9979 g/cc with a percent error of .08, while the known density of water being 0.997041…

repeat 7 a-c 2. Water is formed/drops H2O visible: transfer org layer to dry container before adding drying agent again & let dry again for 15 min. 8. Weigh a 10 ml Erlenmeyer flask/test tube. Transfer the dry methylene chloride sol. With clean, dry pipette to the flask/test tube, leaving drying agent behind. a. 3-4+ spatulafuls of drying agent was added: rinse NaSO4 with approx. 0.5 ml methylene chloride. Stir with dry spatula & transfer to same pre-weighed flask 9. Heat flask in hot water bath…

Dilute the sample by adding distillate water to the cylinder up to 10 cm3. 8. Pipette 2 cm3 of the twice-diluted Vitamin C Fe3+ solution and 2 cm3 of the SCN- and this to a beaker. 9. Fill one of the other cuvettes with this solution. 10. Put the cuvettes in the colorimeter and record the value. Calibrate the colorimeter after every…

It simply used, a test tube, meniscus, and pipette. Then a 15 cm long sprig of elodea was placed in the test tube, after 2 cm was cut from the stem. The test tube was the filled with sodium bicarbonate solution, with 3 cm left at the top of the test tube. The volumeter stopper was then placed in the test tube. The stopper was the adjusted so the solution extended 1 to 2 cm into the pipette. The volumeter test tube apparatus was then left sealed for 10 minutes, in order…

incubated for an additional 24 hours. The 12-well plate was removed from the incubator, and placed in a flume hood. With the use of a P1000 pipette, and carefully making sure not to starch the bottom of the well with the pipette; all the media within the well was removed and disposed in the Waste container. This step was done to each well, with changing the pipette tip each time after disposing the waste. 1 mL of PBS was added per well, once added to all the wells; the PBS was removed and…