Phenols

was negative for sulfur reduction, due to the lack of black precipitate. As well as negative for indole, there was no color change. Lastly, it was negative for motility because there was no growth outside of the initial stab. I next performed the phenol red broth fermentation tests. These were only left in the incubators for twenty-four hours because they produced a result right away. Both broths glucose and lactose had changed to the color yellow. This happened because the ph decreased causing…

it causes a reaction that produce peroxy acid ester and phenol. Peroxy acid can spelled as one word or be called peracid. It is an acid that contains OOH group. The Peroxy acid ester decompose into carbon dioxide (which is a gas that is part of the air we breathe). Phenol is a poisonous crystalline acidic that presents itself as tars of coal and in wood. In dilute solution it is use as an disinfectant. When the peroxy acid ester and phenol mix it releases energy that excites atoms in the…

Methodology 1. Sampling of the material into various study groups and control group. 2. Creating environmental conditions to mimic a forensic scene. 3. Deriving of pulp & storage of samples. 4. Isolation of DNA from pulp using phenol extraction. 5. DNA quantification and quality assessment using nano spectrophotometry. 6. Obtaining primers & its design. 7. Polymerase Chain Reaction & procedure. 8. Result analysis using Gel Electrophoresis. 9. Statistical data analysis 1) Sampling: Extracted…

involved the Mannitol Salt Agar (MSA). MSA is an example of both a selective and a differential media. It selects for halotolerant bacteria and with the aid of phenol-red differentiates for the ability of a microbe to ferment mannitol or not. If a microbe has the ability to ferment mannitol, it produces acidic waste products which react with the phenol-red to yield a yellow color. If the microbe is unable to ferment mannitol, it will consume the proteins present and will produce a pink/red…

result this eliminates E. coli and P. vulgaris. The last test I did was use a Urea broth test for #29. The results from this test were negative which means there was no production of urease to break down urea and produce a pH change to trigger the phenol red to turn pink. This outcome eliminated Klebsiella pneumonia as a possibility. The Urea test indication concluded that the Unknown #29 was Enterobacter aerogenes. But for good measure as a Final test I used the Methyl Red-Vogues Proskauer…

alcohol group; therefore this peak must be corresponded to the O-H stretch of the phenol with a literature value that ranges from 3550-3500 cm-1 (Figure 5). The variance between the observed value, and the expected value can be due to the MHz of the IR spectrometer used. The second peak observed was a weak-medium, sharp and narrow at 1586.79 cm-1. This peak was in agreement with the aromatic C=C stretching of a phenol with a literature value that ranges from 1600-1430 cm-1 (Figure 5). The third…

The NMR sample was prepared by dissolving the collected product in chloroform-D (CDCl3) and DCM for mass spec. The 1H NMR spectra of the 2-bromo-4-methnoxy-phenol product reported significant peaks at (in ppm): δ = 3.02 (s, 2H), 3.75 (s, 4H), 6.97 (1H, dd), 6.81 (1H, dd,), 6.78 (1H, d), 5.15 (1H, s) 3.32 (m, 12H), 3.63 (d, 2H), 3.76 (s, 6H), 3.94 (m, 8H), 5.75 (t, 2H) and 5.82 (t, 2H). 3.78 (3H, s) at (in…

Discussion The T-test from the class supported our hypothesis by showing that Sulfuric Acid diffused significantly faster than phenol red at both temperatures (Fig 1). We can say that our hypothesis for experiment one is supported under certain conditions. The rest of the data in our charts supports our hypothesis as well. However it is not well supported. The rest of the test results were inconsistent with the null hypothesis. The error bars from the class data show a possible cause of…

New ultraviolet (UV) curable prepolymer, 2–hydroxy–3–[p–(1–{2–hydroxy–3–(isopro– phenylcarbonyloxypropoxy) phenyl} methylcyclohexyl) phenoxy] propyl acrylate (BISCHDA) was synthesized by the reaction of p–[1–(p–hydroxyphenyl) methylcyclohexyl] phenol with methylcyclohexanone, epoxidation with epichlorohydrin followed by addition reaction with acrylic acid. All synthesized compounds were characterized by 1H-NMR, 13C-NMR and FT-IR spectroscopy. Several UV-curable formulated solutions were prepared…

of water. Take a small beaker and weigh out about 5 g of ice and pour it into the conical flask having the reaction mixture. (Ice absorbs the heat released during the formation of diazonium compound. It helps to prevent formation of the undesired phenol side product.) Then pour this solution slowly in the sodium nitrite solution, with vigorous stirring. If the solid doesn’t dissolve within 10 minutes. Add another 0.5 g of sodium nitrite. Leave the solution in the ice bath. Part III: Sandmeyer…