Van Tran Microbiology Tuesday 5:00 May 5, 2015 Unknown Sample # 7 1. Introduction: Using biochemical tests are a good way to identify unknown bacteria from a sample. It is basically a similar to a trial and error method, but rather than using trial and error a person would be using positive and negative results. Using biochemical tests will help a person identify certain characteristics of the unknown bacteria through a series of chemical tests. Of course more than one biochemical tests…
Beer is an alcoholic beverage that consist of four main ingredients such as, Water, Barley, and Hops. Yeast was added in the 19th century. Which was an ancient fermentation back then as well after Louis Pasteur identified it as the ancient fermentation way. Although beer is still made from these exact ingredients but this list has never been complete theirs new spices and new ingredients that have been part of the Brew master 's Tool Kit, Including other grains such as sugars, fruits, vegetables…
1.7.3.4. Hydrogen sulphid test (MacFaddine, 1980): Tubes of triple sugar iron agar media were inoculated by suspected culture through stabbing into the butt and the slant then covered with sterile melted paraffin wax and incubated anaerobically for 48 h at 37°C, appearance of blackening in the butt indicates H2S production. 1.7.3.5. Sugar fermentation test (Willis, 1977): Suspected culture was inoculated to 1% peptone water tubes containing 2% bromocresol purple and 1% quantities of the…
The purpose of this experiment was to determine whether there is a correlation between the positive sensitivity to PTC bitterness and the sensitivity to the bitterness of kale. The population sample of twenty-four students was used and a survey. The participants reported on a range was from 0 (not bitter) to 10 (very bitter). A rating of 4 and above would indicate a PTC taster and a high sensitivity to the bitterness of kale. Once the experiment was completed a T-test was applied to the data to…
reduction of expression of the DJ-1 protein was acted upon normal endometrial cells and endometriotic cells through utilization of siRNA was studied. For the purpose of this experiment, several reagents were used such as: DMEM, FCS, antibiotics, and phenol red free DMEM. There were several cells that were utilized such as: stromal cells, endometriotic epithelial cells, and immortalized human endometrial surface epithelial cells. These cells were then injected with the 200 nM DJ-1 SMARTpool…
Lactate dehydrogenase was assayed according to the method of King (1965) [21]. Reagents required: 1. 0.1 M glycine buffer: 7.5 g of glycine and 5.85 mg of sodium chloride were dissolved in one litre of distilled water. 2. Buffered substrate: 2.76 g of lithium lactate was dissolved in 125 ml of glycine buffer containing 75 ml of 0.1 N sodium hydroxide solution. This was prepared just before use. 3. 0.4 N NaOH. 4. 5.0 mg of NAD+ was dissolved in 1.0 ml of distilled water just before use. 5.…
Orbiting spacecraft need a safe, efficient, and cost-effective way of penetrating planetary atmospheres and landing without compromising the cargo or– more importantly– the passengers. In the case of planet Earth, orbiting vehicles generate a substantial amount of thermal energy upon re-entry into the atmosphere, and the management of that immense amount of heat is fundamental in providing a safe environment for the crew and instrumentation on board the vehicle. The design of an efficient and…
vortex [G-560 Scientific Industries, USA] prior to COD test, carbohydrate test and protein assay. The COD in the sludge was measured using spectrophotometer at 620 nm according to the Zhu et al. [11]. The carbohydrate concentration was estimated by the phenol sulphuric acid method, using glucose as a standard [26]. The protein content was analysed using the Lowry protein assay rapid kit, (Wako Co, USA). The NH4+ –N and total nitrogen (TN) and were determined by an auto-analyser TrAACs 8000 using…
rats. Seed extract of Cichorium intybus (CIE) (250mg/kg & 500mg/kg) was administered orally once a day for 3 weeks. Phytochemical investigations of seed extract revealed the presence of some active ingredients such as alkaloids, tannins, saponin, phenols, glycosides, steroids, terpenoids and flavonoids. An elevation of the…
impedes cancer 's spread and speeds tumor destruction while suppressing chronic inflammation. Extracts are effective against colon and bladder cancer, but the flowers that bees source can change honey 's anti-cancer profile. Dark honeys have higher phenol concentrations than other varieties. Raw and locally sourced varieties retain more of their benefits than mass produced…