PH

and 0.1 mL of the enzyme. Each was sat for twenty minutes checking every five minutes for a color change. The only different in this experiment is that one test tube sat in an ice bucket and the other test tube sat in a beaker on a hot plate. For the pH optimum experiment, my partner Gabby and I choose to do two test tube of one having 1.4mL of the very basic, Boric acid, and the other having 1.4mL of the very acid, 5 NHCl. Both test tubes had 0.5 mL of the substrate and 0.1 mL of the enzyme.…

central location of the fish kill site, the salinity level should be between 0.5 to 35 ppt. The typical pH range for an estuarine system is between 5 and 9, which is the range most finfish populations adapt to live in (pH, 2012). The main chemical species responsible for affecting the pH include the levels of oxygen and carbon dioxide (pH, 2012).…

oxidant. Sulfide minerals are the most common producers of AMD, but mining waste rock, tailings, and other mining structures also generate AMD. AMD has a low pH and consists of large concentrations of heavy metal and other toxic elements. This pollutant affects both human health and the environment. AMD also occurs…

Part A: Background Information Acids and bases are very common in today’s society, and they can be found in most households. Acids can be found as by-products of air pollution, concrete cleaner, food flavouring and is produced by the muscle. Bases are found in most cleaning products as ammonia or sodium hydroxide, and they also treat indigestion. There are many ways that acids and bases can be defined by their chemical properties. The Bronsted-Lowry definition states that acids are proton…

Introduction: The primary purpose of this experiment was to find how enzymes affect the reaction rate. During the experiment, factors such as: amount of enzyme, substrate concentration, and pH were tested to find a correlation with enzyme activity. Enzymes, which in the experiment was peroxidase extracted from turnip, are protein catalysts that break up the substrate, which in this experiment was hydrogen peroxide. If there is a correlation between the environmental factors, there should be an…

experiment was to measure how much the pH changes when an individual adds a certain amount of acid or base to water, biological buffers, and other biological solutions. It is vital to study the changes in pH because a slight change in pH in an organism’s body can disrupt metabolism activities and even cause death. To accomplish the purpose of the experiment, an individual will add certain amounts of acid or base to water, biological buffers, or other biological solutions. pH is a measure to…

for pancreatic lipase is lipids or triglycerides. Hydrolysis of triglycerides occurs in the duodenum and the PH rages are from 7.1 to 8.2. • Phenol red was used as a PH indicator of the samples. if the sample is an acid then a yellow color is appeared, if the sample is alkaline then a red color will appeared. if the sample is neutral, it will be orange or if the sample is alkaline and has a PH of 10 or less, then it will have a pink color. Table 1 - Result of Lipid Digestion with…

to test for the most ideal pH and temperature in which catalase could work most efficiently on a substrate. The enzyme for this lab was a catalase located in cow liver and the substrate was hydrogen peroxide (H2O2). We predicted that catalase…

dissociate in water by the following reaction: i) HIn + H2O ⇋ H3O+ + In- The KIn value can be calculated from the reaction: ii) K_In=[H3O+][ In-]/[HIn] Taking the negative logarithm on both sides, iii)〖pK〗_In=pH+log⁡[HIn]/[〖In〗^- ] Solution sample of bromophenol blue with different but similar pH is put into spectrophotometer to find out the ratio of [HIn]/[In-] based on the Beer- Lambert Law1: iv) A=log⁡(l_t/l_o ) Where A is the absorbance of the sample, lt is the intensity of light passed…

separate beakers. 3. Check the pH of both solutions without any NaOH first. Then begin checking pH after 1 drop, then after 10 drops, and lastly 25 drops. After each set of drops be sure to mix solutions. 4. Compare both sets of pH to determine which had an easier time resisting change. C. Titration curves. 1. Insert NaOH into burette, proceed to clamp burette to stand and insert into beaker containing 10 mL of HCl. 2. Add drops of NaOH into the beaker until a change in pH by 0.50 units is seen…