Peroxidase

Hans Zellweger, a Swiss American professor of pediatrics and genetics at the University of Iowa in the mid-1960s described the familial disorder among siblings that was later named after him for the recognition of his discovery, the Zellweger Syndrome. Peroxisomes are cell structures that break down toxic substances and synthesize lipids (fatty acids, oils, and waxes) that are needed for cell function. Peroxisomes are what is needed for normal brain development and its function and it’s what forms the myelin cover which is the substance that coats nerve fibers. Nerve fibers are very thin, thread-like transmission lines that basically carry signals. Responsible for delivering signals and sensations from the nerves to various parts of the human body, such as in the skin, muscles, and internal organs. Now, any one of the 13 genes, termed PEX gene with a defect is a diseased called Zellweger Syndrome. The genes are required for normal formation and function of peroxisome. Peroxisomes are also required in the human body for normal eye, liver, kidney and bone functions. Zellweger syndrome is one of a group of four diseases related and referred to as Peroxisome Biogenesis Disorders (PBD). Toxic molecules that enter the person’s bloodstream fail to be detoxified because of a lack of peroxisome even though there are mechanisms for detoxification. The organic creation of peroxisome plays an important role that’s related to different chemical reactions in a person’s body. Each gene…

6. Quantitative determination of rat peroxisome proliferators activators γ (PPAR-γ): Rat PPAR-γ concentration was determined by ELISA; using a commercially available kit, MyBioSource, Inc., California, USA. The kit utilizing a monoclonal anti- PPAR-γ antibody and an PPAR-γ-HRP conjugate enzyme-linked immunosorbent process assay (ELISA) to determine the concentration of PPAR-γ in serum, plasma, urine, cell culture supernatant and tissue homogenates. Principle of the assay: The sample and buffer…

We placed it in a test tube rack in order to observe the change in a prolonged period of time. We utilized the color palette (Figure 1-A) to see the change in color of the test tube. Since we used an indicator for oxygen, and guaiacol has a high likeness for oxygen, it will bind with oxygen creating a brownish color. Determining the Effect of pH on Enzymatic Activity: This experiment dealt with the effect of pH on peroxidase. First, we made 6 pairs of test tubes. Half of them contained…

environment with a specific set of conditions, other than that it will lead to the denaturation of the enzyme or will render it completely inactive. There are a lot of factors that impact enzyme activities, but in this experiment we examined the effects of 4 different factors which are temperature, Ph level, addition of the inhibitor and enzyme concentration. We supervise the enzyme peroxidase by observing the oxidation of the dye Guaiacol which produces water and a tetraguaiacol which has a…

acids, types of amino acids, and the shape of the string.” Along with this we need to know about a peroxidase; “A peroxidase is one of a number of enzymes that act as catalysts to allow a variety of biological processes to take place. Specifically, they promote the oxidation of various compounds using naturally occurring peroxides, especially…

The purpose of this lab is to find the optimal pH of the enzyme peroxidase, which is found in plant and human cells as a byproduct of photorespiration, photosynthesis or cellular respiration. This is significant because an enzyme operating in nonoptimal pH could have negative effects on the cell, and could pose a health threat to the organism. Enzymes proteins that speed up chemical reactions by lowering the activation energy. They do this by acting as a template for substrate orientation,…

The effect that temperatures at 4°C, 23°C, 32°C, and 48°C , pH 3, 5, 7, and 9, the boiled extract, and hydroxylamine had on the peroxidase enzyme extracted from Brassica rapa. Emma O’Donoghue Fundamentals of Biology I Lab Professor William Olsen October 8, 2015 Abstract The purpose of this lab was to determine the effects that a number of factors had on enzyme activity. The enzyme used during this lab was peroxidase, which was extracted from the organism Brassica rapa. The…

The lack of bubbles was likely caused by the lack of a solid object with oxygen to react to. Yet, this experiment should have yield bubbles as oxygen was still being released. If it were to be boiled, then the amount of bubbles would have heightened, but not by much. If an enzyme is not denatured or inhibited in any fashion or method, it levels off through saturation or continuous use and an abundance of products between enzymes and substrates. To explain the cause of peroxidase becoming…

Horseradish peroxidase (found in the roots of horseradish) is a member of a large family of enzymes known as peroxidases; hemoproteins that can catalyze one-electron oxidation of a substrate in the presence of peroxide. It is largely an alpha-helical protein which binds heme as a redox cofactor. The “heme nitrovinyl” group is specifically where nitrite modification of the heme macrocyle occurs in HRP, in the presence or absence of hydrogen peroxide. It’s regioselective at the 4-vinyl group…

between peroxidase activity and ascorbate content suggests the involvement of ascorbate-peroxidase system in the increased secretion of progesterone resulting from LH action in rabbit. Also the same system renders some evidence for the involvement of this enzyme along with other factors in the process of implantation. Key Words: Reproductive tract, Peroxidase-Ascorbate system Pregnancy & Implantation Introduction: It has been reported that the LH in various species of animals stimulates…