From the experimental results, we have concluded watering rapid-cycling brassica rapa with Gatorade negatively affects growth. The growth difference was very obvious as seen in the results, as the tallest plant water with Gatorade grew only a fifth of what the smallest plant watered with water. However, it is unknown if the Gatorade directly affected the plant or had more affect on the soil. The soil was obviously affected by Gatorade, as the soil became tough and more rock like. The Gatorade…
floor, multiple levels (again, so they can climb), and an exercise wheel so they can run around. Chinchillas are nocturnal, but they will happily wake up if they are getting attention. Their diet should consist of alfalfa hay and chinchilla food pellets, as well as occasional treats, like dandelion drops. They also require a drip bottle filled with distilled water. Their food bowl should be refilled around every 3 days.…
A concise list of steps for preparing a sample for MS analysis is listed below. Step 1. The E. coli pellet with GST-CamK1 construct was resuspended Phosphate Buffered Saline (PBS). Step 2. Sonication was used to lysate the cells. Then, Triton X-100* was added the solution. *Triton X-100 helps solubilize the proteins in cell lysate, but it has to be removed…
Lab 14/ Test 6: Determination of Acid Dissociation Constants through Titration Sabrina Tom I.Abstract The two unknown solutions A and E were titrated with NaOH solutions. For each solution, one fast and three slow titrations were done to see where the pH rose exponentially. The unknown solutions A and E were determined to be Pyridinium ion acid and oxalic acid, respectively. II.Theories One significant concept throughout this lab is titrations. A titration is a lab method analyzes a…
and 1% SDS mixture and 300 μL of 5% potassium acetate and the tubes were stored on ice for 5 minutes. After centrifuging, 600 μL of isopropyl alcohol were added the supernatants of each tube. After cleaning the pellets with 70% ethanol, 45 μL nano pure water was used to dissolve each pellet. Different 30 μL mixtures of pGEM, pGEM-luc, Master Mix N and Master Mix H were incubated at 37°C overnight and kept at 4°C for the following 6…
The death penalty is a type of reward given to criminals who commit serious crimes. The judges are the ones who decide what kind of execution the prisoner gets and what date, although the own criminal can decide for himself too. The judge decides whether or not the criminal gets the death penalty or not. The death penalty has a long history and it has changed over the period of time. Many people approve it while others are against it. Although the death penalty executions cost 1.2 million…
PRE FORMULATION STUDY: Pre formulation testing was an investigation of physical and chemical properties of a drug substance alone and when combined with excipients. It was the first step in the rational development of dosage forms. Class: -Pre formulation study can divide into two subclasses: API characterization, Compatibility study Active pharmaceutical ingredient (API) characterization:- Organoleptic evaluation:-These are preliminary characteristics of any substance which is useful in…
The Holocaust was an awful part of history during 1941-1945. There were concentration camp, some of the really huge ones were, Chelmno, Auschwitz, Belzec, Sobibor, Treblinka, and Maidanek. One that is really noticeable is Auschwitz. This is the most known camp. There were at least 1,100,000 Jews that died. That is not counting all of the homosexuals, gypsies, etc. The Holocaust comes to people's mind and gives people chills. According to Smoke and Ashes by Barbara Rogasky, “the exact number of…
as they are mostly vegetarian. If you have other fish in your tank, be aware that your Oto might not get to the frozen food before any other fish do, as nearly all tropical fish go crazy for these frozen foods. 3. A third great option is algae pellets and flakes. These are convenient to feed and are fairly nutritious. Make sure that the ingredient list has spirulina (algae) near the top, as that is what attracts the catfish and may be the difference between your Oto ignoring the food and eating…
were then centrifuged for 5 minutes at maximum rpm. Almost all the supernatant from each tube was taken out and discarded back into the culture tubes. To each of the pellets in the microfuge tubes, 125 µL of TE buffer were added. With the pipette tip, the pellets were chopped up and mixed by pipetting up and down to resuspend the pellets in the tubes. The two suspensions were then combined into one tube and vortexed vigorously for about 30 seconds. With a P200 micropipette, 60 µL of lysozyme was…