A small burette funnel was filled with column packing solvent at mobile phase, high boiling petroleum, in this case hexane (and then 10%acetone hexane mixture), and Alumina as stationary phase. When adding the sand and the alumina they have to be first mixed with the hexanes in order not to disturb the column. The Lycopene is Red- Orange and more attracted to alumina due to its high degree of Unsaturation, which can be expected to be eluted after Yellow-Orange Carotene pigments. And the other…