Nucleophile

Most of the sPLA2 enzymes possesses a catalytic center that has no catalytic Ser but contains a tightly bound water molecule that acts as a potential nucleophile (Verheij et al., 1980; Scott et al., 1990; Pickard et al., 1996). Majority of eukaryotic sPLA2 also have an N terminal signal peptide for secretion that is cleaved upon internalization into the endoplasmic reticulum or into the periplasm as in…

Assignment 1: Metabolism Gone Wrong: Galactosemia Course: Biochem 3D03 TA: Rabia Mateen Name: Kei Cheng Mak ID: 3122260 Date: October 15th, 2014 Introduction Metabolism consists of biochemical reactions catalyzing the conversion of complex macronutrients into usable energy by various enzymes. Energy in food is extracted from three macronutrients - carbohydrate, protein and fat. As food passes through the digestive tract, various enzymes and chemicals in the body break food particles into…

The hypothesis was supported as the rate of reaction did increase as the concentration and the temperature increased. The concentration, or molarity, of the reactants increased in relation to time in the form M=1.1541e0.0016t. This is an accurate relationship as the volume remains the same as the number of molecules increase, thus the rate would increase exponentially as the molecules collide at a constant growth rate. This is also a reliable model as the R² value is 0.9697. The hypothesis that…

1.5. Enzyme-Polysaccharide Interactions Proteins and enzymes have become an integral part of many industrial processes as well as being applied both in cosmetics and in therapeutics. Due to advances in biotechnology and genetic engineering, mass production of protein and enzymes has become feasible. Use of enzymes is environmentally friendly as they catalyse reactions under mild conditions against polluting inorganic catalysts which works in the extremes of pH and temperature. However, this can…

A small burette funnel was filled with column packing solvent at mobile phase, high boiling petroleum, in this case hexane (and then 10%acetone hexane mixture), and Alumina as stationary phase. When adding the sand and the alumina they have to be first mixed with the hexanes in order not to disturb the column. The Lycopene is Red- Orange and more attracted to alumina due to its high degree of Unsaturation, which can be expected to be eluted after Yellow-Orange Carotene pigments. And the other…