Non-competitive inhibition

constant and the concentration of substrate is increased, the velocity of the reaction will increase rapidly until half of the enzyme becomes saturated with substrate (refer to figure 5). Also, analyzing the graphs suggest that the inhibition type of Cinnamic acid was noncompetitive inhibitor of tyrosinase’s reaction using the Michaelis-Menten and Lineweaver-Burk plots as expectation. As you can see in figure 7, which is Michaelis-Menten plot that showed the “without inhibitor’ and “with inhibitor” curves and you can compared the 2 curve to see if it what type of inhibitor. The graph had 2 sigmoidal curves, 1 is ‘without inhibitor’ and 1 is ‘with inhibitor’. The curve of ‘without inhibitor’ was on top of the ‘with inhibitor’. Since the inhibitor is Cinnamic acid, which is a non-competitive inhibitor according to ncbi.nlm.nih.gov, the ‘with inhibitor’ will be lower and never reach the Vmax of the ‘without inhibitor’ curve. In figure 8, which is the Lineweaver-Burk plot, the graph had 2 linear lines with an equation in a form of y=mx+b. As you can see in the graph, the ‘with inhibitor’ (Cinnamic acid) line was on top of the ‘without inhibitor’. Since it is a non-competitive, the 2 lines did not intersect at y-axis. Also, Cinnamic acid is competitive inhibitor; therefore the Vmax is decrease (#1 Lehninger). This lab used the Lineweaver- Burk plot to calculate the Km and Vmax. The graph was obtained by taking the reciprocal of the Michaelis-Menten Kinetics equation, which give…

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