NMR spectroscopy

To begin the experiment, the Spec 20 was turned on to warm up for 15 minutes and set to maximum absorbance wavelength. Ten test tubes were then prepared. The Spec 20 was set to 0% and a test tube of blank solution (water) was wiped off and placed into the Spec 20. Transmittance was then set to 100% to calibrate the machine. Next, five test tubes were prepared and labeled with one of the standard solutions of 0.05 M, 0.1 M, 0.2 M, and 0.5 M CuSO4 in them. The Spec 20 was set to 600 nm and %…

Introduction: The goal of this lab was for students to learn how to use a spectrophotometer to find the absorbance of four known copper (II) sulfate solutions, four copper (II) sulfate solutions that were created by dilution, and one unknown copper (II) sulfate solution. The purpose of this experiment was to help students learn how to use the Spec-20, learn about the relationship between absorbance and concentration known as the Beer- Lambert law and see the relationship between moles, volume…

A spectroscope takes in light and breaks it into their own individual wavelengths, which are the colored lines we see. The light was produced by electrons, which each have their own quantum, or energy level. When we run a current through the atom, the electron gains energy and jumps to a higher level to its excited state. When they fall back to their ground state, they release their stored energy through the form of light. The line spectra for different atoms differ because they each have a…

technique of spectroscopy; spectroscopy can be defined as the science affiliated with the spectra produced when matter interacts with light. Lab 7 investigation 19, “What Factors Affect the Intensity of Color?” expands on the process of spectroscopy, and its relation to the molarity of a substance. The goal of this lab was to determine the effects of the intensity of color of a solution (2). Spectroscopy is frequently used in the medical field by using the process of infrared spectroscopy, which…

cinnamate, cinnamic acid, and glucose, which are ingredients in the candy, Red Hots. The experiment began with 0.604 grams of a mixture of ethyl cinnamate, cinnamic acid, and glucose. The purity of glucose and ethyl cinnamate was measured through IR Spectroscopy. Melting point was used to determine the purity for cinnamic acid. Using the final amount for each ingredient, we determined the percent yields, which were 64.7% for glucose, 56.2% for cinnamic acid, and 38.8% for ethyl cinnamate. The…

3.4. Friction Characteristics of the NCD, MCD and boron doped NCD Coatings against Alumina Balls The friction coefficient curves of NCD, MCD and boron-doped diamond films sliding with Al2O3 balls are shown in figure 4, figure 5 and figure 6, respectively, for a sliding speed of 8 m/s and a load of 1N, 5N and 10N, respectively. Figure 4 (a), figure 5 (a) and figure 6 (a) show the friction coefficient of NCD, MCD and BDNCD at 1 N, respectively. Figure 4 (b), figure 5 (b) and figure 6 (b) show…

Introduction Quantum dots (Q-Dots) is very small semiconductor particles that means it can be either conductors or resist electricity depending on the temperature and purity of the semiconductor. Q-dots range in size from 2-10 nm, so because of their small size, quantum mechanics governs the physics of the particles. The synthesis Q-Dots were first studied in 1982 by Efros and Ekimov, and then many developments have reported the synthesis of semiconductor nanoparticles.1 Q-Dots have unique…

centrifuged and filtered through 0.45 µm filter, diluted suitably and analysed using UV-Visible spectrophotometer (Systronics, India) at λmax of 227nm. All observations were done in triplicate. Fourier Transform Infrared Spectroscopy (FTIR) Fourier Transform Infrared Spectroscopy was performed to characterize and investigate the interaction between levofloxacin and coformers stearic acid and saccharin sodium used. The FTIR spectra for pure levofloxacin, stearic acid, sodium saccharin, L-ST1 and…

5.2 Discussion 5.2.1 Comparison of the results from AFM and optical tweezers experiments 5.2.1.1 Different unfolding behaviors in AFM and optical tweezers experiments In chapter 3, we have studied AFV3-109 using single-molecule AFM. The results from optical tweezers are contradictory to the results from AFM. First, the unfolding force obtained by optical tweezers is much smaller than the force obtained by AFM. More importantly, in our previous study using AFM, the unfolding of AFV3-109 occurs in…

When EPR spectroscopy is applied, two types of paramagnetic signals have been observed. The low field signal with g = 2.0023 and the other high field signal at g ˜1.96, both are associated with unpaired electrons trapped on oxygen vacancies. It is found that the intensity…