Nitrocellulose

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The SH-SY5Y neuroblastoma cell line was procured from National Centre for Cell Sciences, India and maintained in (DMEM) (Sigma Aldrich, USA) and Ham 's F12 nutrient mixture (F12) (Sigma Aldrich, USA) in 1:1 ratio. The medium was supplemented with 2 mmol L-glutamine (HiMedia, India), 10% (v/v) heat inactivated fetal bovine serum (FBS) (Hyclone Laboratories, Logan, UT) and 1% antibiotics (penicillin/streptomycin). Cells were cultured at 37 ºC in a humidified CO2 ¬incubator containing 5% CO2 and…

2. Materials Haffkine Biopharmaceutical Corporation Limited (HBPCL), Maharashtra, India, made available the venoms of Indian cobra (CV), Common krait (KV), Russell’s viper (RV), and Saw scaled viper (EV) and ASVA. The venoms were distributed in small aliquots, lyophilized, and stored at -20o C. Adult male Swiss albino mice (18 - 20 g) and New Zealand white male rabbits (2.0 - 3.0 kg) were maintained at HBPCL, Maharashtra, India and used for experimentation. Complete Freund 's adjuvant (CFA),…

determined spectrophotometrically using the NanoDrop 2000c spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). Heat-denatured protein samples (300ng per lane) were resolved by SDS-polyacrylamide gel electrophoresis and transferred to nitrocellulose membrane (Boster, Wuhan, China). The membranes were incubated for 60 min in 10% nonfat dry milk to block nonspecific binding, followed by incubation for 12 h at 4℃with primary antibody [primary mouse monoclonal antibody against…

First you start off with an aluminum disc that runs through a machine coating it with the nitrocellulose lacquer, a substance very similar to nail polish. Rollers with scrapers remove the runoff and excess lacquer from the disc and the surplus lacquer is caught in a basin for reuse. The disc hardens fairly quickly similar to nail polish. Often with…

Vinyl Record Materials Materials to used to make records have evolved and changed several times over the years. The earliest disc records from 1889-1894 were made of various materials including wax and. Rubber. Shortly after, a shellac based compound had been experiment with leading to it becoming standard. Each manufactures exact formula varied, but they typically coated records with a shellac (wax) resin Shellac The materials typically used to make for a shellacked disc where cardboard and…

Introduction “Selma,” I think while meaning well, is another piece of counter revolutionary, ruling class propaganda. It is like a “how not to manual” in how not to make revolution, then and now. I was an activist in the days in question in this movie and all the thousands of revolutionary voices that were raised, back in the day, are more thoroughly crushed in this film than all the might of U.S. imperialist military, police, intelligence and public opinion creating machines…