Nitrocellulose

shot shells), and smokeless stick powder (aka extruded powder). The most basic types of smokeless powders are single and double base. The difference between the two is that the single base powder is made from nitrocellulose and the double base is made from a combination of nitrocellulose and nitroglycerin. ‘is there…

their epitope, which is usually five to eight amino acids long on the surface of the protein. Western blot is used to visualize the proteins that will respond to specific antiserum after we separate the proteins. Then place the serums against nitrocellulose…

CRP was identified from patients with acute pneumococcal pneumonia at the Rockefeller Institute for Medical Research (Tillett and Francis, 1930), a name that reflects its reaction with the C-polysaccharide of Pneumococcus. This pentameric protein with MW 118 kDa has five non-covalently bonded and non-glycosylated identical subunits of 206 amino acids each to form a disk-shaped pentagon (Fig. 1). CRP has been proven as an early indicator of infectious or inflammatory conditions as well as a…

Preparation for Cell Lysis Obtain the mammalian expression vector for homo sapien ESR2 from Addgene. Assemble the vector to code for the proteins snail, slug and twist. Transfect HEK293 cells with the modified plasmids in petri dishes. Allow the cells to grow for 1-2 days so they will express the proteins. Transfer the culture medium to a centrifuge tube to separate it from the cells. Add 1mL of phosphate buffered saline (PBS) to the cells and swirl to mix. Separate the PBS from the cells and…

Christian Friedrich Schonbein One of the more obscure scientists, Christian Friedrich Schonbein was well known for his discovery of ozone as well as work on fuel cells. He along with Friedrich Goppelsroeder discovered and coined capillary analysis, and later on, paper chromatography. My project centers on ink chromatography and capillary action, both of which were discovered by Friedrich Goppelsroeder and Christian Friedrich Schonbein. Of the two men, Christian Friedrich Schonbein was a more…

and oils from plants and cleaning the remnants of metal and glass, so why often a cleaner or ingredient is found 1.2.3. as a solvent Botanone is an effective and common solvent used in processes which involving gums, resins, cellulose acetate, nitrocellulose, and vinyl films. For this reason, it finds its use in the plastic and textile industry, in the production of paraffin wax, and in household products such as varnish, varnish, paint remover, change agent for deformation of alcohol, glue,…

flammableliquid with a mildly pungent and somewhat aromatic odour. It is miscible in all proportionswith water and with organic solvents such as ether, methanol, ethyl alcohol, and esters.Acetone is used as a solvent for cellulose acetate and nitrocellulose, as a carrier for acetyleneand as a raw material for the chemical synthesis of a wide range of products such as ketene,methyl methacrylate, bisphenol A, diacetone alcohol mesityl oxide, methyl isobutyl ketone,hexylene glycol ( 2-methyl-2,…

An experiment was performed to analyze the effects of heat shock in yeast called Saccharomyces cerevisiae, to assess the hypothesis that yeast extracts up-regulate gene expression during heat shock the same way bacteria cells up-regulate gene expression during heat shock. In bacteria cells, heat shock causes the bacteria to up-regulate gene expression and produce more proteins. Similarly, gene expression and protein production increases in yeast during heat shock. Therefore, the experiment was…

2. Materials and methods 2.1. Chemicals Zingerone was obtained from Alfa Aesar, Heysham, UK. Cisplatin, Bradford reagent, trichloroacetic acid (TCA), thiobarbituric acid (TBA), reduced glutathione (GSH), glacial metaphosphoric acid (Gmpa), 5,5ˊ-dithiobis-(2-nitrobenzoic acid) (DTNB), proteinase K and agarose were purchased from Sigma-Aldrich Co, MO, USA. All other chemicals and solvents used were of the highest purity grade available. 2.2. Animals Sixty Adult male albino rats (180-200g) were…

extraction and immunoblotting were then done to prepare the adhesion assay. The cells were lysed and the proteins were eluted through boiling in 3X SDS sample buffer and then the amino concentration was calculated. The proteins were placed in a nitrocellulose membrane, in which they were then ready for immunoblotting. GFP as well as other materials were used as the antibodies for conjugation. The bands were then observed. In order to do the cell…