bacteria from other bacterias the following medium was used: MSA plate, SIM plate, nitrate reduction test Nutrient gelatin and Urease broth. For Metabolic tests, only Catalase test were performed on the unknown bacteria. These tests were best use gram negative bacteria to indicated what the unknown microbe is. For the Catalase test, the use of hydrogen preoxide on the sample of the unknown…
from Loba Chemie. The β-mercatpoethanol (mol. wt. 78.13) from LOBA Chemie, sodium dodecyl sulfate (SDS) (mol. wt. 288.38) and urea (mol. wt. 60.06) was purchased from Merck, and ethanol was purchased from Sigma-Aldrich. Antimicrobial strain of gram negative bacteria Escherichia coli (E. coli) [MTCC 118] and gram positive bacteria Bacillus subtilis (B. subtilis) [MTCC 121] was purchased from MTCC Chandigarh, India. 2.1. Fabrication of Porous Scaffold from Keratin/Alginate binary blend: The…
essential (Bush 2014). One recent study shows 50% of HIV/AIDS patients had severe sepsis at ICU admission or during the ICU stay. An astounding 90% of nosocomial infections were the source of sepsis and the microbiology of infections was mostly gram-negative rods Serratia marcescens (three) made up 7% (Japiassú…
In this experiment, three strains of tetracycline resistant E. Coli were tested. First, the bacteria were diluted via serial dilution in order to more accurately count and quantify the bacteria. To ensure that the three different strains of bacteria are not cross contaminated, label everything either Farm A, Farm B, or Farm C depending on what is being tested. There are six agar plates for each farm, three of the plates are tetracycline plates (which are labeled) and three are unlabeled. For…
Rhetorical Situation Analysis Report A Universal Basic Income Would Insure Against Job Loss 1. This text originally appears in “The New York Times” room for debate. It was updates on October 4, 2016. 2. The genre for this text is an opposite of editorial (also known as op- ed). The rhetorical aim for this genre is persuasive. More specifically, to Propose a Solution. This style focuses on a question about what action can be taken. Stern’s article talks about how the United States needs to…
Velocity of Bacteria and Cells Determine the Need of Antibiotics The first goal was to examine the motion of E. coli bacteria. Excel was used to analyze and record the position, velocity and acceleration of the bacteria. The second goal was to analyze and compare the cell motion of wound healing, neutrophils and E.coli bacteria using ImageJ to determine if a patient needs to take antibiotics to cure his injury based on the velocity of E. coli, neutrophils, and wound healing. Experimental…
Desulfovibrio is gram negative bacteria. There are media that will be useful in isolating this organism. The best option for a gram negative bacteria would be using a selective media specifically MacConkey agar or EMB agar. These agars are used to isolate gram negative bacteria because the crystal violet in the MacConkey agar will not allow the growth of gram positive bacterium making it a good media to isolate Desulfovibrio. If that selective media does not work then another media is to use a…
Hankin performed an interesting experiment to test if the antibacterial ‘substance’ in Ganges and Jamuna was volatile in nature. He heated the Ganges and Jamuna water in an open and a hermatically sealed tube (Hankin, 1896 and Hankin 2011- this is the English version of the original French paper translated by gangagen.com). He found that the antibacterial activity was retained when the water was heated in the sealed tube, but was lost in the open tube. Although the exact temperature and the…
As transdermal delivery is a non-invasive route for the therapeutic materials delivery from the intact skin towards the systemic circulation. And as every square centimeter of the surface of the human skin contains ≈10–70 hair follicles and ≈200–250 sweat glands, hence the skin surface could be a conduit for the delivery of therapeutic agents. Additional benefits of using transdermal route is being a painless delivery method, few side effects, providing a low-frequency of therapy administration,…
Type IV pili are a family of appendage protein filaments that are assembled on the surfaces of many different microorganisms, such as bacteria and archea. The wide range of diverse microorganisms within the type IV pili family indicates that there must be both a common evolutionary origin, as well as a versatile functionality (Giltner, Nguyen and Burrows 2012). The formation of type IV pili occurs when the enzyme PilD, first recognized in the bacterial species Pseudomonas aeruginosa, catalyzes…